ECM550
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
동의어(들):
Basement membrane cell invasion assay
크기 선택
제품정보 (DICE 배송 시 비용 별도)
제품 이름
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric, The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
species reactivity
vertebrates
manufacturer/tradename
Chemicon®
QCM
technique(s)
cell based assay: suitable
detection method
colorimetric
shipped in
wet ice
Quality Level
Application
Cell Structure
Disclaimer
General description
Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit′s performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.
Application:
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Other Notes
Cell Stain: (Part No. 20294) One 10 mL bottle.
Cotton Swabs: (Part No. 10202) 24 each.
Forceps: (Part No. 10203) One each.
Packaging
Preparation Note
Legal Information
저장 등급
10-13 - German Storage Class 10 to 13
wgk
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Cell migration is stimulated and directed by interaction of cells with the extracellular matrix (ECM), neighboring cells, or chemoattractants. Cell migration participates in morphogenic processes, wound healing and tumor metastasis. Specifically, inhibiting tumor invasion by blocking tumor cell chemotaxis has been a major focus of research. Tumor cell invasion, marked by degradation of ECM, is also directly correlated with metastatic potential.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| ECM550 | 04053252325052 |
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