biological source
Staphylococcus aureus
form
lyophilized
specific activity
~15,000 U/mg
packaging
pkg of 15,000 U
manufacturer/tradename
Roche
optimum pH
7.5
shipped in
wet ice
Application
- Removal of endogenous RNA from in vitro translation systems
- RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.
Biochem/physiol Actions
Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.
Preparation Note
Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Organ-specific translation elongation rates measured by in vivo ribosome profiling.
Gerashchenko M V, et al.
bioRxiv, 279257 (2018)
High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
Sequence specific cleavage of African green monkey ?-satellite DNA by micrococcal nuclease.
Horz W, et al.
Nucleic Acids Research, 11(13), 4275-4285 (1983)