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Merck

67309

Kovac′s reagent for indoles

suitable for microbiology

동의어(들):

4-(Dimethylamino)benzaldehyde solution

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C9H11NO
CAS 번호:
100-10-7
Molecular Weight:
149.19
UNSPSC Code:
41171621
NACRES:
NA.85
PubChem Substance ID:
329760541
MDL number:
MFCD00003381
Beilstein/REAXYS Number:
4132845

주요 문서

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InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

SMILES string

[H]C(=O)c1ccc(cc1)N(C)C

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

agency

according to ISO 16654:2001

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

4-(dimethlyamino)benzaldehyde, 50 g/L , hydrochloric acid, 240 g/L , isoamylic alcohol, 710 g/L

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages, microbiology

storage temp.

2-8°C

suitability

Escherichia coli, coliforms

Quality Level

200

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관련 카테고리

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

저장 등급

3 - Flammable liquids

wgk

WGK 3

flash_point_f

109.4 °F

flash_point_c

43 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
BCCN2957
BCCL5138
BCCK4337
BCCH8824
BCCG3914

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Rita R. Colwell, R. Grigorova
Methods in Microbiology, 19, 27-27 (1987)
S. Harisha
An Introduction to Practical Biotechnology, 193-193 (2005)
Patrick R Porubsky et al.
Archives of biochemistry and biophysics, 475(1), 14-17 (2008-04-22)
Cytochrome P450 2A13 (CYP2A13) is a lung specific enzyme known to activate the potent tobacco procarcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) into two carcinogenic metabolites. CYP2A13 has been crystallized and X-ray diffraction experiments illuminated the structure of this enzyme, but with an unknown
R W Trepeta et al.
Journal of clinical microbiology, 19(2), 172-174 (1984-02-01)
Escherichia coli is the most common gram-negative microbe isolated and identified in clinical microbiology laboratories. It can be identified within 1 h by oxidase, indole, lactose, and beta-glucuronidase tests. The oxidase and indole tests are performed as spot tests, and
C Creuzenet et al.
The Journal of biological chemistry, 275(45), 34873-34880 (2000-08-24)
FlaA1 is a small soluble protein of unknown function in Helicobacter pylori. It has homologues that are essential for the virulence of numerous medically relevant bacteria. FlaA1 was overexpressed as a histidine-tagged protein and purified to homogeneity by nickel chelation
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