grade
Molecular Biology
Quality Level
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
pH
6.5-7.2
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.
Application
LBBroth (Miller) is suitable for:
- non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
- selective cultivation when appropriate antibiotics are added.
- the cultivation of E-coli cells for anti-bacterial assays.
Features and Benefits
Miller′s LB liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Other Notes
10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
10 g/L NaCl
5 g/L Yeast Extract
저장 등급
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
프로토콜
General protocols for growth of competent cells in microbial medium.
문서
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
유전 공학 및 복제의 발전은 연구 목적을 위한 이형 Protein Expression Systems 및 분리에 수많은 가능성을 열어주었습니다. 상당한 수준의 기술 발전으로 재조합형 단백질의 대규모 발현과 분리가 가능하게 되었습니다.
Long-term retention of hydrophilic behavior of plasma treated polydimethylsiloxane (PDMS) surfaces stored under water and Luria-Bertani broth
Zhao LH, et al.
Sensors and actuators A, Physical, 181.0, 33-42 (2012)
Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50
Antonio Basanta, Beatriz Gomez-Sala, et al.
Biotechnology, 76, 3314-3324 (2010)
Andrada-Ioana Damian-Buda et al.
Journal of functional biomaterials, 13(4) (2022-10-25)
Mesoporous bioactive glass nanoparticles (MBGNs) are widely recognized for their ability to bond to hard tissue, while the ions released from the BG structure enhance specific cellular pathways. In this study, the SiO2-P2O5-CaO-MgO-ZnO system was used to successfully synthesize MBGNs