A2429
Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
동의어(들):
Goat Anti-Mouse IgG (Fc specific)–AP
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB CL
Citations:
45
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
should not react with
bovine, human, horse
technique(s)
direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): 1:160,000-1:320,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Immunogen
Purified mouse IgG, Fc fragment
Application
Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1500) and western blot. The antibody can also be used for immunohistochemistry (1:40 using formalin-fixed, paraffin-embedded sections).
Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA), sandwich ELISA and enzyme-linked immune absorbent spot (ELISPOT).
The product has been used as a secondary antibody for detecting mouse serum anti-hemagglutinin (HA) antibodies using ELISA techniques.
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with bovine, horse, or human samples.
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Antibody structure, instability, and formulation
Wang W, et al.
Journal of Pharmaceutical Sciences, 91(6), 252-265 (2007)
Pedro Humberto Castro et al.
Journal of experimental botany, 69(19), 4633-4649 (2018-07-28)
Post-translational modifiers such as the small ubiquitin-like modifier (SUMO) peptide act as fast and reversible protein regulators. Functional characterization of the sumoylation machinery has determined the key regulatory role that SUMO plays in plant development. Unlike components of the SUMO
Release of Staphylococcus aureus extracellular vesicles and their application as a vaccine platform.
Xiaogang Wang et al.
Nature communications, 9(1), 1379-1379 (2018-04-13)
Secretion of extracellular vesicles (EVs), a process common to eukaryotes, archae, and bacteria, represents a secretory pathway that allows cell-free intercellular communication. Microbial EVs package diverse proteins and influence the host-pathogen interaction, but the mechanisms underlying EV production in Gram-positive
Marcel Zocher et al.
International immunology, 15(7), 789-796 (2003-06-17)
Antigen-specific B cells are key players in many autoimmune diseases through the production of autoreactive antibodies that can cause severe tissue damage and malfunction. We have designed and expressed a fusion protein, referred to as MOG-Fc, composed of the extracellular
The specificity of targeted vaccines for APC surface molecules influences the immune response phenotype
Grodeland G, et al.
Testing, 8(11), e80008-e80008 (2013)
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Instructions
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