A2554
Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
동의어(들):
Goat Anti-Mouse IgG (Fc specific)–HRP
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB CL
Citations:
167
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
should not react with
bovine, human, horse
technique(s)
direct ELISA: 1:70,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL)
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Application
Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in:
- immunohistochemistry
- enzyme-linked immunosorbent assay (ELISA)
- immunoblot
- dot-blot analysis
Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.
Biochem/physiol Actions
Goat anti-mouse IgG(Fc specific)-peroxidase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no reactivity with mouse Fab fragment, human IgG, IgA, IgM, κ and λ light chain, bovine IgG and IgM, or horse IgG.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization and antibody dependent cell mediated cytotoxicity.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Preparation Note
Adsorbed to reduce background staining with bovine, horse, or human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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