A4418
Ammonium sulfate
Molecular Biology, ≥99.0%
동의어(들):
Mascagnite
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
Linear Formula:
(NH4)2SO4
CAS 번호:
Molecular Weight:
132.14
PubChem Substance ID:
eCl@ss:
38030408
UNSPSC Code:
12352314
NACRES:
NA.31
EC Number:
231-984-1
MDL number:
Assay:
≥99.0%
Grade:
Molecular Biology
Form:
powder
Solubility:
H2O: 0.667 g/mL
InChI key
BFNBIHQBYMNNAN-UHFFFAOYSA-N
InChI
1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)
grade
Molecular Biology
assay
≥99.0%
form
powder
technique(s)
affinity binding assay: suitable, protein purification: suitable
impurities
≤0.005% Insolubles
pH
5.0-6 (25 °C, 132 g/L)
mp
>280 °C (dec.) (lit.)
solubility
H2O: 0.667 g/mL
density
1.77 g/mL at 25 °C (lit.)
anion traces
chloride (Cl-): ≤5 ppm
cation traces
Cu: ≤5 ppm, Fe: ≤5 ppm, Zn: ≤5 ppm, heavy metals (as Pb): ≤2 ppm
absorption
≤0.025 at 280 at 1 M, ≤0.03 at 260 at 1 M
suitability
suitable for flow cytometry, suitable for molecular biology
application(s)
metabolomics
microbiology
foreign activity
DNase, RNase, and protease, none detected
Quality Level
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관련 카테고리
General description
Ammonium sulfate is a versatile inorganic sulfate salt, plays crucial role in biochemical research, molecular biology, and cell culture. It serves as an indispensable tool for purifying antibodies and precipitating or fractionating proteins. Furthermore, it is instrumental in crystallographic analysis of nucleic acids and proteins and also used as a nitrogen supplement in synthetic laboratory media. Our premium-grade Ammonium Sulfate is meticulously crafted to be DNase, RNase, and Protease-free, ensuring its suitability for cutting-edge research.
Application
Ammonium sulfate helps in quick precipitation of celluar proteins. Ammonium sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins.Ammonium sulfate has been used
- in the preparation of ′Blue Silver′ colloidal Coomassie stain for proteins.
- to precipitate the Shiga-like toxin from the culture supernatant.
- for precipitaion of viral particles from the culture supernatant.
Features and Benefits
- Heavy metals (as Pb): ≤2 ppm
- Free from DNase, RNase, and protease
Other Notes
For additional information on our range of Biochemicals, please complete this form.
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Assessment of Cell Cycle and Induction of Apoptosis by Shiga-like Toxin Produced by Escherichia coli O157:H7 in T47D Breast Cancer Cells Using Flow Cytometry
Wayan Suardana et al.
Asian Pacific Journal of Cancer Prevention, 23-10, 3247-3252 (2022)
Use of ammonium sulphate as a sulphur fertilizer: Implications for ammonia volatilization
David S Powlson, Chris J Dawson
Soil use and management, 38(1), 622-634 (2022)
Protein precipitation using ammonium sulfate
P Wingfield
Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 3 (2001)
William P Heal et al.
Nature protocols, 7(1), 105-117 (2011-12-24)
A protocol for selective and site-specific enzymatic labeling of proteins is described. The method exploits the protein co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carbon saturated fatty acid) to an N-terminal glycine catalyzed by the enzyme
Mohd Baasir Gaisawat et al.
Microorganisms, 8(1) (2020-01-08)
Clostridium (C.) difficile-infection (CDI), a nosocomial gastrointestinal disorder, is of growing concern due to its rapid rise in recent years. Antibiotic therapy of CDI is associated with disrupted metabolic function and altered gut microbiota. The use of probiotics as an
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Product Information Sheet
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