Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge.
Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs.

purified rabbit IgG

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications.

Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody.

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Immunoglobulins are glycoprotein antibodies that modulate several immune responses. Immunoglobulin G participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Specificity of the Peroxidase Conjugated Anti-Rabbit IgG antibodies is determined by immunoelectrophoresis (IEP) versus normal rabbit serum and rabbit IgG.

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Prepared by two-step glutaraldehyde method.

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