C3306
Calcium chloride dihydrate
Molecular Biology, ≥99.0%
동의어(들):
CaCl2.2H2O, Calcium chloride, Calcium dichloride
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
실험식(Hill 표기법):
CaCl2 · 2H2O
CAS 번호:
Molecular Weight:
147.01
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12352302
EC Number:
233-140-8
MDL number:
Assay:
≥99.0%
Grade:
Molecular Biology
Form:
powder
Solubility:
H2O: 294 mg/mL, clear, colorless
InChI key
LLSDKQJKOVVTOJ-UHFFFAOYSA-L
InChI
1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
SMILES string
O.O.Cl[Ca]Cl
grade
Molecular Biology
assay
≥99.0%
form
powder
mp
176 °C (dec.) (lit.)
solubility
H2O: 294 mg/mL, clear, colorless
foreign activity
DNase, RNase, protease, none detected
Quality Level
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Application
Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)
Biochem/physiol Actions
Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
저장 등급
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Preparation of calcium competent Escherichia coli and heat-shock transformation
Chang AY, et al.
Journal of Experimental Microbiology and Immunology, 1, 22-25 (2017)
Bradley T Estes et al.
Nature protocols, 5(7), 1294-1311 (2010-07-03)
The ability to isolate, expand and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, as well
Hui Sun Leong et al.
Nature communications, 5, 3947-3947 (2014-05-24)
Non-coding RNAs (ncRNAs) are frequent and prevalent across the taxa. Although individual non-coding loci have been assigned a function, most are uncharacterized. Their global biological significance is unproven and remains controversial. Here we investigate the role played by ncRNAs in
Zhijian Zhao et al.
Scientific reports, 10(1), 4399-4399 (2020-03-12)
The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of
Denisa Hathazi et al.
The EMBO journal, 39(23), e105364-e105364 (2020-11-01)
Reversible infantile respiratory chain deficiency (RIRCD) is a rare mitochondrial myopathy leading to severe metabolic disturbances in infants, which recover spontaneously after 6-months of age. RIRCD is associated with the homoplasmic m.14674T>C mitochondrial DNA mutation; however, only ~ 1/100 carriers develop
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