HEPES solution

HEPES has been used as a component of:

• Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices
• Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity
• cell culture media to provide buffer capacity to maintain the pH of the cell line
• buffer in decellularization of porcine nucleus pulposus

Concentrations of HEPES over 40mM may be toxic to some cell types.

HEPES Buffer Solution, a sterile-filtered 1M solution of 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a vital biological buffer in cell biology, biochemical, and biological research. This zwitterionic buffer enjoys widespread adoption due to its effective pH range of 6.8 to 8.2, making it suitable for a diverse array of research applications. HEPES excels as a buffering agent in cell culture media, offering several advantages over alternatives like Tris and phosphate. Notably, HEPES exhibits lower cell toxicity, ensuring optimal cell health and viability during experiments.

Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields.

It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

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Prepared from HEPES free acid and cell culture grade water.