H0887
HEPES solution
1M, liquid, pH 7.0-7.6, suitable for cell culture, BioXtra, BioReagent
동의어(들):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12161704
PubChem Substance ID:
NACRES:
NA.75
MDL number:
eCl@ss:
32129211
제품 이름
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
SMILES string
OCCN1CCN(CCS(=O)(O)=O)CC1
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
sterility
sterile-filtered
product line
BioReagent, BioXtra
assay
0.9—1.1 M
form
liquid
storage condition
(Tightly closed. Dry), dry at room temperature
concentration
1 M
Quality Level
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
colorless
pH
7.0-7.6
useful pH range
6.8-8.2
7.5
density
1.1 g/cm3
suitability
suitable for component for culture media, suitable for electrophoresis
application(s)
cell analysis
life science and biopharma
sample preparation
storage temp.
2-8°C
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General description
HEPES Buffer Solution, a sterile-filtered 1M solution of 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a vital biological buffer in cell biology, biochemical, and biological research. This zwitterionic buffer enjoys widespread adoption due to its effective pH range of 6.8 to 8.2, making it suitable for a diverse array of research applications. HEPES excels as a buffering agent in cell culture media, offering several advantages over alternatives like Tris and phosphate. Notably, HEPES exhibits lower cell toxicity, ensuring optimal cell health and viability during experiments.
Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields.
Furthermore, it surpasses bicarbonate buffers (NaHCO3) in its ability to maintain physiological pH despite fluctuations in carbon dioxide concentration arising from cellular respiration, a key factor in maintaining cellular homeostasis. Beyond its exceptional performance in cell culture, HEPES may also find application in various biological and biochemical studies. It may also be valuable in techniques like immunoprecipitation, cell lysis, and live cell imaging, providing a stable and controlled environment for experimental procedures. The combination of its versatility, exceptional pH buffering capacity, and minimal interaction with other molecules positions HEPES as an indispensable tool across diverse research fields.
It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used as a component of:
- Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices
- Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity
- cell culture media to provide buffer capacity to maintain the pH of the cell line
- buffer in decellularization of porcine nucleus pulposus
Features and Benefits
- High purity product for biochemical and biological research
- Sterile filtered and cell culture tested
- Ready Solution reduces the need
- Useful for the pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
- Ready available solution reduce the need for preparation time
Preparation Note
Prepared from HEPES free acid and cell culture grade water.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Disclaimer
Concentrations of HEPES over 40mM may be toxic to some cell types.
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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