M2786
3-Maleimidobenzoic acid N-hydroxysuccinimide ester
crystalline
๋์์ด(๋ค):
MBS
์กฐ์ง ๋ฐ ๊ณ์ฝ ๊ฐ๊ฒฉ์ ๋ณด๋ ค๋ฉด ๋ก๊ทธ์ธ๋ฅผ ํด๋ฆญํฉ๋๋ค.
ํฌ๊ธฐ ์ ํ
์ ํ์ ๋ณด (DICE ๋ฐฐ์ก ์ ๋น์ฉ ๋ณ๋)
์คํ์(Hill ํ๊ธฐ๋ฒ):
C15H10N2O6
CAS ๋ฒํธ:
Molecular Weight:
314.25
UNSPSC Code:
12352108
NACRES:
NA.22
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1505254
Form:
crystalline
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๋์ ๋ฌธ์InChI key
LLXVXPPXELIDGQ-UHFFFAOYSA-N
SMILES string
O=C1CCC(N1OC(C2=CC=CC(N3C(C=CC3=O)=O)=C2)=O)=O
InChI
1S/C15H10N2O6/c18-11-4-5-12(19)16(11)10-3-1-2-9(8-10)15(22)23-17-13(20)6-7-14(17)21/h1-5,8H,6-7H2
form
crystalline
reaction suitability
reagent type: cross-linking reagent
mp
175-177 ยฐC (lit.)
solubility
DMSO: ≤20 mg/mL
functional group
NHS ester, maleimide
storage temp.
−20ยฐC
Quality Level
๊ด๋ จ ์นดํ ๊ณ ๋ฆฌ
General description
may require addition of solvent to coupling buffer to at least 5% to maintain solubility
Application
3-Maleimidobenzoic acid N-hydroxysuccinimide ester (MBS) has been used as a protein cross-linker. It has been used for the stabilization of actin filaments in plant tissue samples.
3-Maleimidobenzoic acid N-hydroxysuccinimide ester has been used:
- in the fixation of pollen in Arabidopis
- as a component of Murashige and Skoog (MS) medium to stabilize F-actin in Eucalyptus root hairs
- in the fixation of F-actin labelled pollen tubes of Lilium davidii
Heterobifunctional crosslinking reagent reactive toward primary amine and sulfhydryl. Typically, coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.0, followed by coupling to compounds containing sulfhydryl by thioether at same pH. Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates.
Biochem/physiol Actions
3-Maleimidobenzoic acid N-hydroxysuccinimide is used as a fixative for the preservation of pollen tube F-actin distribution. It imposes less damage when used on pollen tubes.
Other Notes
The aryl maleimide is less stable than alkyl maleimide linkers.
์ ์ฅ ๋ฑ๊ธ
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
๊ฐ์ฅ ์ต์ ๋ฒ์ ์ค ํ๋๋ฅผ ์ ํํ์ธ์:
์ด ์ ํ์ ์ด๋ฏธ ๊ฐ์ง๊ณ ๊ณ์ญ๋๊น?
๋ฌธ์ ๋ผ์ด๋ธ๋ฌ๋ฆฌ์์ ์ต๊ทผ์ ๊ตฌ๋งคํ ์ ํ์ ๋ํ ๋ฌธ์๋ฅผ ์ฐพ์๋ณด์ธ์.
Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes
Qu X, et al.
Bio-protocol, 10(01), 204-211 (2020)
Andrii Slonchak et al.
BMC genomics, 20(1), 474-474 (2019-06-12)
Extracellular vesicles (EVs) are small membrane vesicles secreted by the cells that mediate intercellular transfer of molecules and contribute to transduction of various signals. Viral infection and action of pro-inflammatory cytokines has been shown to alter molecular composition of EV
Comparison of F-actin fluorescent labeling methods in pollen tubes of Lilium davidii
Wang L, et al.
Plant Cell Reports, 24(5), 266-270 (2005)
Ilenia Papa et al.
Nature, 547(7663), 318-323 (2017-07-13)
Protective high-affinity antibody responses depend on competitive selection of B cells carrying somatically mutated B-cell receptors by follicular helper T (T
Jeong Hwan Seong et al.
Genes, 9(7) (2018-07-04)
The 14-3-3 family of proteins performs key regulatory functions in phosphorylation-dependent signaling pathways including cell survival and proliferation, apoptosis, regulation of chromatin structure and autophagy. In this study, the zeta isoform of 14-3-3 proteins (designated as Tm14-3-3ζ) was identified from
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