TOX1

In Vitro Toxicology Assay Kit, MTT based

Name: <I>In Vitro</I> Toxicology Assay Kit, MTT based
Brand: SIGMA

동의어(들):

mitochondrial activity assay

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:

NA.84

UNSPSC Code:

12352207

주요 문서

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기술 서비스

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도움 문의

기술 서비스

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도움 문의

usage

kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λ_max

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

2-8°C

Quality Level

200

General description

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Application

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Biochem/physiol Actions

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

pictograms

GHS02,GHS08,GHS05,GHS07,GHS09

signalword

Danger

hcodes

H225,H314,H335,H336,H341,H410

pcodes

P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

target_organs

Central nervous system, Respiratory system

저장 등급

3 - Flammable liquids

flash_point_f

53.6 °F

flash_point_c

12 °C

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시험 성적서(COA)

Lot/Batch Number

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