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MABC1614-25UG Anti-AATF Antibody, clone 1B2D8

Detect AATF using this rat monoclonal Anti-AATF, clone 1B2D8 Antibody, Cat. No. MABC1614-25UG, validated for use in Western Blotting and Immunocytochemistry.

Anti-AATF Antibody, clone 1B2D8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer UniProt Number: Q9JKX4
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MABC1614-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1614-25UG
      DescriptionAnti-AATF Antibody, clone 1B2D8
      Alternate Names
      • Protein AATF
      • Apoptosis-antagonizing transcription factor
      • Rb-binding protein Che-1
      • Traube protein
      Background InformationProtein AATF (UniProt: Q9JKX4; also known as Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, Traube protein) is encoded by the Aatf (also known as Che1, Trb) gene (Gene ID: 56321) in murine species. AATF is a nuclear protein that functions as a general inhibitor of the histone deacetylase HDAC1. It binds to the pocket region of RB1 to displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. AATF is expressed in adrenal gland, Purkinje cells, heart, kidney, liver, lung, muscle, ovary, and testis. It is expressed uniformly throughout the embryo until E10.5. However, from E11.5, the relative expression level increases in the liver, hind brain, spinal cord, dorsal root ganglia, and the posterior commissure. Down-regulation of AATF is involved in colon carcinoma cell proliferation and over-expression of Aatf gene interferes with MAP3K12 induced apoptosis.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect AATF using this rat monoclonal Anti-AATF, clone 1B2D8 Antibody, Cat. No. MABC1614-25UG, validated for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected AATF in Immunocytochemistry applications (Ishigaki, S., et. al. (2010). Cell Death Differ. 17(5):774-86).

      Western Blotting Analysis: A representative lot detected AATF in Western Blotting applications (Ishigaki, S., et. al. (2010). Cell Death Differ. 17(5):774-86).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the first 39 amino acids from murine Apoptosis-antagonizing transcription factor (AATF).
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 1B2D8 is a rat monoclonal antibody that detects AATF in murine and human cells. It targets an epitope within the first 39 amino acids from the N-terminal region.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected AATF in 10 µg of  HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1614-25UG 04054839437908