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DR1086 Anti-ATF3 Mouse mAb (6B8)

Anti-ATF3, mouse monoclonal, clone 6B8, recognizes the human ATF3 protein. It is validated for ELISA and Western blotting.

Anti-ATF3 Mouse mAb (6B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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DR1086
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ATF3 protein.
      Catalogue NumberDR1086
      Brand Family Calbiochem®
      Application Data
      Detection of human ATF3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human ATF3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-ATF3 Mouse mAb (6B8) (Cat. No. DR1086). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human ATF3 (aa 1-182), expressed as a GST fusion protein.
      Clone6B8
      HostMouse
      IsotypeIgG₃
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1086 0

      Documentation

      Anti-ATF3 Mouse mAb (6B8) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ATF3 Mouse mAb (6B8) Certificates of Analysis

      TitleLot Number
      DR1086
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human ATF3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human ATF3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-ATF3 Mouse mAb (6B8) (Cat. No. DR1086). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ATF3 protein.
      BackgroundActivating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (δZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
      HostMouse
      ImmunogenFull-length, recombinant, human ATF3 (aa 1-182), expressed as a GST fusion protein.
      Clone6B8
      IsotypeIgG₃
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review