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ABE2614 Anti-ATF5

Anti-ATF5, Cat. No. ABE2614, is a highly specific rabbit polyclonal antibody that targets Cyclic AMP-dependent transcription factor ATF-5 and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-ATF5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ATFS, ATFX UniProt Number: Q9Y2D1
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ABE2614
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Mk, R, HIF, ICC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2614
      DescriptionAnti-ATF5
      Alternate Names
      • Cyclic AMP-dependent transcription factor ATF-5
      • cAMP-dependent transcription factor ATF-5
      • Activating transcription factor 5
      • Transcription factor ATFx
      Background InformationCyclic AMP-dependent transcription factor ATF-5 (UniProt: also known as cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx) is encoded by the ATF5 (also known as ATFX) gene (Gene ID: 22809) in human. ATF5 belongs to the ATF/CREB family of transcription factors that is widely expressed. It has been implicated in multiple biological processes, such as regulation of cell proliferation and differentiation, cell stress response and survival. ATF5 can either stimulate or repress gene transcription through binding of different DNA regulatory elements such as CRE (cAMP response element), ARE (ATF5 regulatory element), and AARE (amino acid response element). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the pericentriolar material (PCM). ATF5 depletion is reported to block PCM accumulation at the centrosome and cause fragmentation of centrioles, which contributes to the formation of multi-polar mitotic spindles and genomic instability. ATF5 homeostasis is essential for the function of the centrosome and for the global microtubule organization.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ATF5, Cat. No. ABE2614, is a highly specific rabbit polyclonal antibody that targets Cyclic AMP-dependent transcription factor ATF-5 and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected ATF5 in 10 µg of C6 cell lysate.

      Western Blotting Analysis: A representative lot detected ATF5 in Western Blot applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92).

      Immunofluorescence Analysis: A representative lot detected ATF5 in Immunofluorescence applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92).

      Western Blotting Analysis: A representative lot detected ATF5 in transfected Hela Cells (Courtesy of Dr. David X. Liu from Washington State University).

      Immunofluorescence Analysis: A representative lot detected ATF5 in Cos-7, Hela, and C6 cells (Courtesy of Dr. David X. Liu from Washington State University).
      Biological Information
      ImmunogenA linear peptide corresponding to 18 amino acids from the N-terminal half of human Cyclic AMP-dependent transcription factor ATF-5.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets a sequence in the N-terminal region and detects ATF5 by immunocytochemstry.
      Species Reactivity
      • Monkey
      • Rat
      • Human
      Species Reactivity NoteHuman, Monkey, Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ATFS
      • ATFX
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~32 kDa observed; 30.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in COS-7 cells.

      Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected ATF5 in COS-7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2614 04054839156458