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MAB1012-I-25UG Anti-Alkaline Phosphatase Antibody, E. coli

Anti-Alkaline Phosphatase, E. coli, Cat. No. MAB1012-I, is a mouse monoclonal antibody that detects Alkaline Phosphatase and is tested for use in Western Blotting.

MAB1012-I-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1012-I-25UG
      DescriptionAnti-Alkaline Phosphatase Antibody, E. coli
      Alternate Names
      • EC:3.1.3.1
      • b0383
      • JW0374
      • alkaline phosphomonoesterase
      • glycerophosphatase
      • phosphomonoesterase
      Background InformationAlkaline phosphatase (UniProt: P00634; also known as EC:3.1.3.1, APase) is encoded by the phoA (also known as b0383, JW0374) gene (Gene ID: 945041) in E. coli. Alkaline phosphatase is a dimeric, non-glycosylated metalloenzyme, requiring two zinc ions (Zn²⁺) and one magnesium ion (Mg²⁺) per subunit for its activity and structural stability. The active site of Alkaline phosphatase includes a serine residue that plays a crucial role in its catalytic mechanism. The primary function of E. coli Alkaline phosphatase is to catalyze the hydrolysis of phosphate monoesters, resulting in the release of an inorganic phosphate and an alcohol. The enzyme is involved in various processes, including phosphate metabolism and regulation. Alkaline phosphatase is also known for its ability to transfer phosphate groups to acceptor molecules, which is key in its role in transphosphorylation reactions. Alkaline Phosphatase has a wide range of applications in molecular biology. It is used as a reporter enzyme in various assays, including ELISA and Western blotting. During molecular cloning, it is commonly used to dephosphorylate the 5' ends of DNA fragments to prevent self-ligation during cloning procedures.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Alkaline Phosphatase, E. coli, Cat. No. MAB1012-I, is a mouse monoclonal antibody that detects Alkaline Phosphatase and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected Alkaline Phosphatase in Western Blotting application (Yogev, D., et al. (1991). J Bacteriol. 173(6):2035-44; Taw, M.N., et al. (2022). Sci Rep. 12(1):14862).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenPurified Alkaline Phosphatase from E. coli.
      EpitopeUnknown
      CloneN/A
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityThis mouse recombinant monoclonal antibody detects Alkaline Phosphatase from E. coli.
      IsotypeIgG2bκ
      Species Reactivity
      • E. coli
      Species Reactivity NoteE. Coli.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~50 kDa observed. 49.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from E. coli.

      Western Blotting Analysis: A 1:1,000 Dilution of this antibody detected Alkaline Phosphatase in E. coli lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1012-I-25UG 04065272290326

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