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MAB4619 Anti-Bcl-XL Antibody, NT, clone 7D9

This Anti-Bcl-XL Antibody, N-terminus, clone 7D9 is validated for use in FC, IF for the detection of Bcl-XL.

Anti-Bcl-XL Antibody, NT, clone 7D9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BCL2L1, BCLX, bcl-xL, DKFZp781P2092, BCL-XL/S, Bcl-X, BCL2L, bcl-xS UniProt Number: Q07817
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MAB4619
50 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4619
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Bcl-XL Antibody, NT, clone 7D9
      Background InformationBcl-X consists of two isoforms, Bcl-XL (long), a 241 aa protein, and Bcl-XS (short), a 178 aa protein lacking a 63-aa domain that is well conserved among members of the Bcl-2 family. Bcl-XL represses cell death, while Bcl-XS favors cell death.Bcl-X has two isoforms, Bcl-XL (long) a 241 amino acid protein and Bcl-XS (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family. Bcl-XL represses cell death, while Bcl-XS favors cell death.
      References
      Product Information
      FormatPurified
      PresentationImmunoglobulin purified by protein A affinity chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Bcl-XL Antibody, N-terminus, clone 7D9 is validated for use in FC, IF for the detection of Bcl-XL.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      Application NotesImmunofluorescence

      Flow Cytometry

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 35-50 (TEAPEETEAERETPSA) of mouse Bcl-XL protein. This sequence is conserved at 15 of 16 residues in rat Bcl-XL.
      EpitopeN-terminus
      Clone7D9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB4619 recognizes a protein of 27 kDa, identified as the Bcl-XL protein. Highly specific to Bcl-XL and shows no cross-reaction with Bcl-XS, Bcl-2alpha or Bax protein.
      IsotypeIgG2a
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.
      Gene Symbol
      • BCL2L1
      • BCLX
      • bcl-xL
      • DKFZp781P2092
      • BCL-XL/S
      • Bcl-X
      • BCL2L
      • bcl-xS
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q07817 # Potent inhibitor of cell death. Isoform Bcl-X(L) anti- apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.
      SIZE: 233 amino acids; 26049 Da
      SUBUNIT: Bcl-X(L) forms homodimers, and heterodimers with BAX, BAK and BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Also interacts with BAD and BBC3. Isoform Bcl-X(L) binds to Siva isoform 1. Interacts with BCL2L11 (By similarity). Interacts with BECN1 and PGAM5.
      SUBCELLULAR LOCATION: Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus envelope; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity).
      TISSUE SPECIFICITY: Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
      DOMAIN: SwissProt: Q07817 The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
      PTM: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
      SIMILARITY: Belongs to the Bcl-2 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4619 04053252502637

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies