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MABF2089-25UG Anti-CD28 Antibody, clone 37.51

This syrian hamster monoclonal Anti-CD28, clone 37.51 Antibody, Cat. No. MABF2089 is tested for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Affects Function, for the detection of CD28.

Anti-CD28 Antibody, clone 37.51 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Cd28 UniProt Number: P31041
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MABF2089-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFC, WB, IP, FUNCSHmPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2089-25UG
      DescriptionAnti-CD28 Antibody, clone 37.51
      Alternate Names
      • T-cell-specific surface glycoprotein CD28
      Background InformationT-cell-specific surface glycoprotein CD28 (UniProt: P31041, also known as CD28) is encoded by the Cd28 gene (Gene ID: 12487) in murine species. CD28 is a single-pass type I membrane protein that exists as a disulfide linked homodimer. In human CD28 is expressed by a majority of T cells in the periphery, including all CD4+ cells and about 50% CD8+ cells. CD28 is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 2-150), a transmembrane domain (aa 151-177), and a cytoplasmic domain (aa 178-218). CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. It enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. B7-1 (CD80) and B7-2 (CD86) are two known ligands of CD28 on activated B cells and antigen presenting cells (APC). These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction co-stimulatory signaling between the B7 ligands and CD28 is essential for crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
      References
      Product Information
      FormatPurified
      PresentationPurified syrian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
      Applications
      ApplicationThis syrian hamster monoclonal Anti-CD28, clone 37.51 Antibody, Cat. No. MABF2089 is tested for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Affects Function, for the detection of CD28.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunoprecipitation
      • Affects Function
      Application NotesAffects Function Analysis: A representative lot detected CD28 in Affects Function applications (Ulges, A., et. al. (2015). Nat Immunol. 16(3):267-75; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65; Brender, C., et. al. (2007) Blood. 1;110(7):2528-36).

      Flow Cytometry Analysis: A representative lot detected CD28 in Flow Cytometry applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65).

      Immunoprecipitation Analysis: A representative lot detected CD28 in Immunoprecipitation applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8).

      Western Blotting Analysis: A representative lot detected CD28 in Western Blotting applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8
      Biological Information
      ImmunogenEL-4 T lymphoma cells.
      Clone37.51
      ConcentrationPlease refer to lot specific datasheet.
      HostSyrian Hamster
      SpecificityClone 37.51 is a Syrian hamster monoclonal antibody that detects CD28 in murine cells.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cd28
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight25.24 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in EL-4 T lymphoma cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected CD28 in 1X10E6 EL-4 T lymphoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2089-25UG 04054839529566