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MABC1774-25UL Anti-COPS5 Antibody, clone 12-12E

Anti-COPS5, clone 12-12E, Cat. No. MABC1774, is a mouse monoclonal antibody that detects COPS5 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MABC1774-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1774-25UL
      DescriptionAnti-COPS5 Antibody, clone 12-12E
      Alternate Names
      • COP9 signalosome complex subunit 5
      • SGN5
      • Signalosome subunit 5
      • Jun activation domain-binding protein 1
      Background InformationCOP9 signalosome complex subunit 5 (UniProt: Q92905; also known as SGN5, Signalosome subunit 5, Jun activation domain-binding protein 1, COPS5) is encoded by the COPS5 (also known as CSN5, JAB1) gene (Gene ID: 10987) in human. COPS5 is the fifth component of the COP9 signalosome (CSN) complex that directly interacts with and regulates the activity of cellular and developmental processes, including checkpoint control, DNA repair, apoptosis, and cell cycle control. This complex is an essential regulator of the ubiquitin conjugation pathway that mediates the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to a reduction in ubiquitin ligase activity of SCF-type complexes. In the CSN complex, COPS5 probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. However, it lacks the metalloproteinase activity and requires the other subunits of CSN complex. COPS5 is present both in the nucleus and cytoplasm. Its nuclear localization is reduced in the presence of interferon induced protein with tetratricopeptide repeats 3 (IFIT3). COPS5 contains a JAMM motif (aa 138-151) that is shown to be essential for the protease activity of the CSN complex. Higher levels of COPS5 are observed in a variety of human cancers and are correlated with a poor prognosis. (Ref.: Kato, J-Y., et al (2006). Hybridoma. 25(6); 342-348).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-COPS5, clone 12-12E, Cat. No. MABC1774, is a mouse monoclonal antibody that detects COPS5 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected COPS5 in NIH3T3 cell lysate.

      Immunocytochemistry Analysis: A representative lot detected COPS5 in Immunocytochemistry applications (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).

      ELISA Analysis: A representative lot detected COPS5 in ELISA applications (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).

      Western Blotting Analysis: A representative lot detected COPS5 in Western Blotting applications (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated COPS5 in Immunoprecipitation applications (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).

      Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected COPS5 in NIH3T3 cell line.

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected COPS5 in Immunohistochemistry applications (Kato, J.Y., et. al. (2006). Hybridoma. 25(6):342-8).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to the first 198 amino acids from human COP9 signalosome complex subunit 5 (COPS5).
      EpitopeN-terminal
      Clone12-12E
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 12-12E is a mouse monoclonal antibody that detects COP9 signalosome complex subunit 5 (COPS5). It targets an epitope within the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COPS5
      • CSN5
      • JAB1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed; 37.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected COPS5 in HEK293T cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1774-25UL 04065265944687