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DR1105 Anti-CRY1 Mouse mAb (4H4-1C4)

This Anti-CRY1 Mouse mAb (4H4-1C4) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of CRY1.

Anti-CRY1 Mouse mAb (4H4-1C4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cryptochrome 1

DR1105
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~60-65 kDa CRY1 protein in HeLa NE cells and human colon adenocarcinoma tissue.
      Catalogue NumberDR1105
      Brand Family Calbiochem®
      SynonymsAnti-Cryptochrome 1
      Application Data
      Detection of human CRY1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CRY1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (10 µg/ml). Detection: fluorescence.

      Detection of human CRY1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (5 µg/ml). Detection: colorimatric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa NE cells, Human colon adenocarcinoma tissue
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (1- µg/ml)
      Paraffin Sections (5 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human CRY1 (aa 1-587), expressed as a GST fusion protein
      Clone4H4-1C4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1105 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-July-2010 RFH
      SynonymsAnti-Cryptochrome 1
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (1- µg/ml)
      Paraffin Sections (5 µg/ml)
      Application Data
      Detection of human CRY1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human CRY1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (10 µg/ml). Detection: fluorescence.

      Detection of human CRY1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue. Primary antibody: Anti-CRY1 Mouse mAb (4H4-1C4) (Cat. No. DR1105) (5 µg/ml). Detection: colorimatric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~60-65 kDa CRY1 protein.
      HostMouse
      ImmunogenFull-length, recombinant, human CRY1 (aa 1-587), expressed as a GST fusion protein
      Clone4H4-1C4
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa NE cells, Human colon adenocarcinoma tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review