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MABE1103 Anti-CST complex subunit CTC1 Antibody, clone C482

Anti-CST complex subunit CTC1, clone C482, Cat. No. MABE1103, is a highly specific mouse monoclonal antibody that targets CTC1 and has been tested in and Western Blotting.

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MABE1103
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1103
      DescriptionAnti-CST complex subunit CTC1 Antibody, clone C482
      Alternate Names
      • CST complex subunit CTC1
      • Conserved telomere maintenance component 1
      • HBV DNAPTP1-transactivated protein B
      Background InformationCST complex subunit CTC1 (UniProt Q2NKJ3; also known as Conserved telomere maintenance component 1, HBV DNAPTP1-transactivated protein B) is encoded by the CTC1 (also known as C17orf68, CRMCC) gene (Gene ID 80169) in human. CTC1 is a subunit of the trimeric CST (CTC1, STN1, and TEN1) complex that binds ssDNA, promotes telomere DNA synthesis, and inhibits telomerase-mediated telomere elongation. CST directly interacts with and stimulates DNA polymerase -primase (pol -primase) activity. CST also associates with and sequesters telomeric 3' overhangs after their elongation by telomerase, thereby limiting further telomere extension by telomerase. Knockdown of either STN1 or CCT1 in human cancer cells and in mouse fibroblasts results in substantially elongated telomeric G-ove+D13rhangs. Conditional deletion of CTC1 in mice leads to early entry into senescence and signs of telomeric replication defects, Human CTC1 gene mutations casue dyskeratosis congenita (DC) and related bone marrow failure syndromes, as well as the rare autosomal recessive neurological disorder Coats Plus, where patients develop cranial calcifications and cysts. Coats Plus and DC patients develop premature hair graying, anemia, and osteoporosis as a result of telomerase defects.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CST complex subunit CTC1, clone C482, Cat. No. MABE1103, is a highly specific mouse monoclonal antibody that targets CTC1 and has been tested in and Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.75-1 µg/mL from a representative lot detected the exogenously overexpressed CTC1 in 100-150 ng of lysate from transfected HeLa cells, as well as downregulated CTC1 expression following conditional CTC1 gene knockout in 150 ng of lysate from a HCT116 cell line harboring floxed CTC1 alleles (Courtesy of Carolyn Price, Ph.D. and Xuyang Feng, Ph.D., University of Cincinnati College of Medicine, U.S.A.).
      Biological Information
      ImmunogenLinear peptide corresponding to an N-terminal region sequence of human CTC1.
      CloneC482
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone C482 targets an N-terminal region sequence present in both human CTC1 spliced isoforms reported by UniProt (Q2NKJ3).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTC1
      • C17orf68
      • CRMCC
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~135 kDa observed. 134.61/114.1 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIdentity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2b .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1103 04054839132926

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