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MAC148-25UG Anti-Cas9 Antibody, clone 8G4

Anti-Cas9, clone 8G4, Cat. No. MAC148, is a rat monoclonal antibody that detects Cas9 and is tested for use in Western Blotting and Immunofluorescence.

MAC148-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAC148-25UG
      DescriptionAnti-Cas9 Antibody, clone 8G4
      Alternate Names
      • CRISPR-associated endonuclease Cas9/Csn1
      • SpCas9
      • SpyCas9
      Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt: Q99ZW2; also known as SpCas9, SpyCas9, Cas9) is encoded by the Cas9 gene (Gene ID: 69900935) in Streptococcus pyogenes. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements, and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. (Ref.: Fonfara, I., et al. (2014). Nucleic Acids Res. 42(4); 2577-2590; Jinek, M., et al. (2012). Science. 337(6096); 816-821).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Cas9, clone 8G4, Cat. No. MAC148, is a rat monoclonal antibody that detects Cas9 and is tested for use in Western Blotting and Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Cas9 in Cell line stably expressing Cas9-NLS (A) versus wild type parental cell line (B) (Courtesy of Germán Camargo Ortega (Institute of Stem Cell Research, Helmholtz Munich,. Germany).

      Western Blotting Analysis: A representative lot detected Cas9 in Western Blotting application. (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged, full-length, recombinant Cas9 from Streptococcus pyogenes.
      EpitopeUnknown
      Clone8G4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 8G4 is a rat monoclonal antibody that specifically detects Cas9.
      IsotypeIgG2aλ
      Species Reactivity
      • All
      Species Reactivity NoteAll.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cas9
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~200 kDa observed; 158.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells overexpressing Cas9.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Cas9 in lysate from HeLa cells overexpressing Cas9.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAC148-25UG 04065271175112