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AM47 Anti-Caspase-9 (Ab-2) Mouse mAb (1-2)

Anti-Caspase-9 (Ab-2) Mouse mAb (1-2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-APAF-3, Anti-CASP9, Anti-Mch6, Anti-ICE-LAP6

AM47
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~46 kDa latent form of caspase-9 in HeLa or Jurkat cells.
      Catalogue NumberAM47
      Brand Family Calbiochem®
      SynonymsAnti-APAF-3, Anti-CASP9, Anti-Mch6, Anti-ICE-LAP6
      References
      ReferencesCardone, M.H., et al. 1998. Science 282, 1318.
      Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Hakem, R., et al. 1998. Cell 94, 339.
      Hu, Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 4386.
      Kuida, K, et al. 1998. Cell 94 325.
      Perkins, C., et al. 1998. Cancer Res. 58, 4561.
      Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
      Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
      Positive controlHeLa or Jurkat cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Not Frozen Sections
      Not Immunofluorescence
      Not Immunoprecipitation
      Not Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of an amino terminal fragment of human caspase-9
      ImmunogenHuman
      EpitopeNear the N-terminus
      Clone1-2
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM47 0

      Documentation

      Anti-Caspase-9 (Ab-2) Mouse mAb (1-2) Certificates of Analysis

      TitleLot Number
      AM47

      References

      Reference overview
      Cardone, M.H., et al. 1998. Science 282, 1318.
      Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Hakem, R., et al. 1998. Cell 94, 339.
      Hu, Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 4386.
      Kuida, K, et al. 1998. Cell 94 325.
      Perkins, C., et al. 1998. Cancer Res. 58, 4561.
      Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
      Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      SynonymsAnti-APAF-3, Anti-CASP9, Anti-Mch6, Anti-ICE-LAP6
      ApplicationImmunoblotting (1 µg/ml)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~46 kDa latent form of caspase-9.
      BackgroundCaspase-9 (ICE-LAP6, MCH-6, APAF-3) is involved in the early activation cascade that occurs following an apoptotic signal. Caspase-9 exists as a pro-form (m.w. aproximately 46 kDa) that is cleaved by the activity of APAF-1 in the presence of cytosolic cytochrome c (APAF-2) and dATP. The processing of pro-caspase-9 into a heterodimer consisting of a 35 kDa and a 10 kDa chain, activates the protease and induces the cascade leading to cleavage of capase-3 and other apoptotic targets. Caspase-9 is also cleaved by that activity of caspase-8 and caspase-10 and also by caspase-3 in a feedback loop. The processing of caspase-9 may be blocked by the activity of bcl-x, which physically interacts with the caspase-9/APAF-1 complex. Processing of caspase-9 is also regulated by the phosphorylation of casapse-9. Mutation of the caspase-9 gene or caspase-9 knockouts results in embryonic lethality due to an enlarged and malformed cerebrum and defective brain development resulting from decreased apoptosis.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of an amino terminal fragment of human caspase-9
      EpitopeNear the N-terminus
      Clone1-2
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlHeLa or Jurkat cells
      Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCardone, M.H., et al. 1998. Science 282, 1318.
      Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
      Hakem, R., et al. 1998. Cell 94, 339.
      Hu, Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 4386.
      Kuida, K, et al. 1998. Cell 94 325.
      Perkins, C., et al. 1998. Cancer Res. 58, 4561.
      Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
      Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.