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324402 Anti-Dopamine D₃ Receptor Rabbit pAb

Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.

Anti-Dopamine D₃ Receptor Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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324402
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Pricing & Availability

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324402-100UL
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      		 Plastic ampoule 100 ul
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      Description
      OverviewRecognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors.
      Catalogue Number324402
      Brand Family Calbiochem®
      References
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat brain
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (see comments)
      Flow Cytometry (1:100, see application references)
      Immunoblotting (1:500)
      Immunohistochemistry (1:5000)
      Application CommentsThis antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      324402-100UL 04055977215854

      Documentation

      Anti-Dopamine D₃ Receptor Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dopamine D₃ Receptor Rabbit pAb Certificates of Analysis

      TitleLot Number
      324402

      References

      Reference overview
      Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-July-2007 RFH
      ApplicationELISA (see comments)
      Flow Cytometry (1:100, see application references)
      Immunoblotting (1:500)
      Immunohistochemistry (1:5000)
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D3 dopamine receptor protein.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
      IsotypeIgG
      Specieshuman, rat
      Positive controlRat brain
      FormLyophilized
      FormulationUndiluted serum.
      PreservativeNone
      CommentsThis antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
      Toxicity Standard Handling
      ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
      Application referencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.