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MABF1973-25UL Anti-Etanercept Antibody, clone ETA63C8

This mouse monoclonal Anti-Etanercept, clone ETA63C8 Antibody, Cat. No. MABF1973-25UL is validated for use in Neutralizing and ELISA, for the detection of Etanercept.

Anti-Etanercept Antibody, clone ETA63C8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology
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MABF1973-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HNEUT, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF1973-25UL
      DescriptionAnti-Etanercept Antibody, clone ETA63C8
      Alternate Names
      • Enbrel®
      Background InformationEtanercept is a chimeric fusion protein consisting of the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1. This Fc component contains the hinge, CH2 and CH3 regions, but not the CH1 region of IgG1. Etanercept binds to TNF-alpha and blocks its interactions with receptors. Hence, it is considered a TNF inhibitor that functions as a decoy receptor that binds to TNF. Although it mimics the inhibitory effects of naturally occurring soluble TNF receptors, the difference is that Etanercept has a greatly extended half-life in the bloodstream. Its speci fic activity is reported to be about 1.7 x 106 units/mg. Etanercept is shown to modulate biological responses produced by TNF, including expression of adhesion molecules responsible for leukocyte migration, serum levels of cytokines, and serum levels of matrix metalloproteinase-3. It is also shown to affect animal models of inflammation, including murine collagen-induced arthritis. Clone ETA63C8 can bind to Etanercept and block its activity.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationThis mouse monoclonal Anti-Etanercept, clone ETA63C8 Antibody, Cat. No. MABF1973-25UL is validated for use in Neutralizing and ELISA, for the detection of Etanercept.
      Key Applications
      • Neutralizing
      • ELISA
      Application NotesNeutralizing Analysis: A representative lot neutralized Etanercept in Neutralizing applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).

      ELISA Analysis: A representative lot detected Etanercept in ELISA applications (Detrez, I., et. al. (2016). J Immunol. 196(6):2879-84).

      ELISA Analysis: 4 µg/mL from a representative lot detected Etanercept in human serum with AS (ankylosing spondylitis) (Courtesy of Professor Ann Gils, Ph.D., KU Leuven Belgium).
      Biological Information
      ImmunogenChimeric fusion protein cotaining the extracellular ligand-binding portion of the p75 TNF receptor linked to the Fc portion of human IgG1.
      CloneETA63C8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone ETA63C8 detects Etanercept in human samples. It can bind to Etanercept and block its activity.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight150 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.
      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF1973-25UL 04054839456190