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MABC1145 Anti-FAP Antibody, clone 73.3

Detect fibroblast activation protein using this mouse monoclonal Anti-FAP Antibody, clone 73.3, Cat. No. MABC1145. It has been tested in Flow Cytometry.

Anti-FAP Antibody, clone 73.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Fap UniProt Number: P97321
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MABC1145
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MFCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1145
      DescriptionAnti-FAP Antibody, clone 73.3
      Alternate Names
      • Fibroblast activation protein
      • Propyl endopeptidase FAP
      • Dipeptidyl peptidase FAP
      • Serine integral membrane protease
      • SIMP
      Background InformationFibroblast activation protein (UniProt: ; also known as Propyl endopeptidase FAP, Dipeptidyl peptidase FAP, Serine integral membrane protease, SIMP) is encoded by the Fap gene (Gene ID: 14089) in murine species. FAP is a homodimeric type II transmembrane protein that physically attaches to cells and bulk of the protein is exposed to extracellular space. It is a gelatinase of the serine protease family that is selectively expressed by tumor associated fibroblasts and pericytes in a majority of human epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. It is not expressed in most normal tissues, but is expressed during embryonic development. It is present as a homodimer and homodimerization is required for activity of both plasma membrane and soluble forms. It can also form heterodimer with DPP4. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Its gelatinase activity is inhibited by several serine-protease inhibitors, such as PMSF, AEBSF, and diisopropyl fluorophosphate (DFP). FAP is involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. It is also involved in the cell invasiveness towards the ECM in malignant melanoma cancers. (Ref.: Kelly, T et al. (2012). Int. Rev. Cell Mol. Biol. 297:83-116; Santos, AM et al. (2009). J. Clin. Invest. 119(12):3613-3625).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect fibroblast activation protein using this mouse monoclonal Anti-FAP Antibody, clone 73.3, Cat. No. MABC1145. It has been tested in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A representative lot detected FAP in Flow Cytometry applications (Wang, L.C., et. al. (2014). Cancer Immunol Res. 2(2):154-66).

      Flow Cytometry Analysis: 1 µg/mL from a representative lot detected FAP in 3T3/FAP overexpressed cells, but not in 3T3 WT cells. (Courtesy of Leslie Hopper, University of Pennsylvania, USA).
      Biological Information
      ImmunogenFAP-expressing 3T3 cells.
      Clone73.3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 73.3 specifically detects fibroblast activation protein (FAP) in 3T3 cells overexpressing FAP.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Fap
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight87.94 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.


      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1145 04054839169601

      Documentation

      Anti-FAP Antibody, clone 73.3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-FAP Antibody, clone 73.3 Certificates of Analysis

      TitleLot Number
      Anti-FAP, clone 73.3 - 3197663 3197663
      Anti-FAP, clone 73.3 - 3291375 3291375
      Anti-FAP, clone 73.3 - 3670127 3670127
      Anti-FAP, clone 73.3 - 3862615 3862615
      Anti-FAP, clone 73.3 - 3874286 3874286
      Anti-FAP, clone 73.3 - 3959465 3959465
      Anti-FAP, clone 73.3 - 3977212 3977212
      Anti-FAP, clone 73.3 - 4070059 4070059
      Anti-FAP, clone 73.3 - 4097740 4097740
      Anti-FAP, clone 73.3 - 4176612 4176612
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