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DR1016 Anti-H2AX Rabbit pAb

Anti-H2AX Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Histone H2AX

DR1016
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form in 293T and A431 cell nuclear extracts.
      Catalogue NumberDR1016
      Brand Family Calbiochem®
      SynonymsAnti-Histone H2AX
      Application Data
      Detection of human H2AX by immunoblotting. Sample: Nuclear extract from HEK293T cells, H2AX knockout (-/-), H2AX wild-type (+/+), and G-361 cells. Primary antibody: Anti-H2AX Rabbit pAb (Cat. No. DR1016) (1:1000). Detection: chemiluminescence.
      References
      ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
      Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
      Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
      Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
      Product Information
      FormLiquid
      FormulationIn Tris/citrate-phosphate buffer, pH 7-8.
      Positive controlNuclear extracts from human 293T or G-361 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:5000-1:15,000)
      Immunoprecipitation (5-20 µg/mg protein from lysate)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human H2AX
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1016 0

      Documentation

      Anti-H2AX Rabbit pAb Certificates of Analysis

      TitleLot Number
      DR1016

      References

      Reference overview
      Stiff. T., et al. 2004. Cancer Res. 64, 2390.
      Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
      Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
      Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      SynonymsAnti-Histone H2AX
      ApplicationImmunoblotting (1:5000-1:15,000)
      Immunoprecipitation (5-20 µg/mg protein from lysate)
      Application Data
      Detection of human H2AX by immunoblotting. Sample: Nuclear extract from HEK293T cells, H2AX knockout (-/-), H2AX wild-type (+/+), and G-361 cells. Primary antibody: Anti-H2AX Rabbit pAb (Cat. No. DR1016) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquitinated form.
      BackgroundThe nucleosome is composed of ~200 bp of DNA and eight histone proteins, two from each of the four histone protein families, H4, H3, H2B, and H2A. H2A includes three subfamilies: H2A1-H2A2, H2AZ, and H2AX (LocusLink ID 3014), which have been conserved throughout eukaryotic evolution. Phosphorylation of H2AX on Ser139 is an early step in the response to DNA damage induced by ionizing radiation. Phosphorylation of H2AX is required for the accumulation of 53BP1 at DNA break sites.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human H2AX
      IsotypeIgG
      Specieshuman, mouse
      Positive controlNuclear extracts from human 293T or G-361 cells
      FormLiquid
      FormulationIn Tris/citrate-phosphate buffer, pH 7-8.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Highly Toxic
      ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
      Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
      Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
      Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.