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DR1014 Anti-HAUSP Rabbit pAb

Anti-HAUSP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Herpes-Associated Ubiquitin-Specific Protease, Anti-USP7

DR1014
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~135 kDa HAUSP protein in HeLa cell nuclear extracts.
      Catalogue NumberDR1014
      Brand Family Calbiochem®
      SynonymsAnti-Herpes-Associated Ubiquitin-Specific Protease, Anti-USP7
      Application Data
      A. Detection of human HAUSP by immunoblotting. Sample: Whole cell extracts from HeLa cells (50 µg). Primary antibody: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (1:3000) Detection: chemiluminescence B. Detection of human HAUSP by immunoprecipitation followed by immunoblotting. Sample: Nuclear extract from HeLa cells (1 mg). Antibody for IP: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (2 µg/mg lysate). Immunoblotting conditions: Same as panel A with 10 µg HeLa nuclear extract used as the control.
      References
      ReferencesLi, M., et al. 2004. Mol. Cell 13, 879.
      Muyang, L., et al. 2002. Nature 416, 648.
      Vugmeyster, Y., et al. 2002. Mol. Immunol. 39, 431.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlHeLa cell nuclear extracts
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000-1:10,000)
      Immunoprecipitation (1-4 µg/mg lysate)
      Application CommentsHAUSP protein is reported to stabilize p53 through the deubiquitination of p53. HAUSP has also been reported to be cleaved by caspase-3. May recognize additional bands, possibly corresponding to HAUSP cleavage products. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids encoded within exon 3 of human HAUSP
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1014 0

      Documentation

      Anti-HAUSP Rabbit pAb Certificates of Analysis

      TitleLot Number
      DR1014

      References

      Reference overview
      Li, M., et al. 2004. Mol. Cell 13, 879.
      Muyang, L., et al. 2002. Nature 416, 648.
      Vugmeyster, Y., et al. 2002. Mol. Immunol. 39, 431.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      SynonymsAnti-Herpes-Associated Ubiquitin-Specific Protease, Anti-USP7
      ApplicationImmunoblotting (1:1000-1:10,000)
      Immunoprecipitation (1-4 µg/mg lysate)
      Application Data
      A. Detection of human HAUSP by immunoblotting. Sample: Whole cell extracts from HeLa cells (50 µg). Primary antibody: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (1:3000) Detection: chemiluminescence B. Detection of human HAUSP by immunoprecipitation followed by immunoblotting. Sample: Nuclear extract from HeLa cells (1 mg). Antibody for IP: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (2 µg/mg lysate). Immunoblotting conditions: Same as panel A with 10 µg HeLa nuclear extract used as the control.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~135 kDa HAUSP protein.
      Backgroundp53, a tumor suppressor phosphoprotein is present at low concentrations in normal cells. The low concentration is maintained by MDM2-mediated ubiquitination and resulting proteolysis. HAUSP, an ubiquitin hydrolase, is thought to regulate MDM2 and help stabilize p53. When cells are subjected to stress, such as hypoxia or UV radiation, p53 is activated, its protein levels are elevated and its ubiquitin-dependent degradation is blocked. The resulting increase in p53 leads either to the induction of cell cycle arrest or apoptosis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids encoded within exon 3 of human HAUSP
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cell nuclear extracts
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsHAUSP protein is reported to stabilize p53 through the deubiquitination of p53. HAUSP has also been reported to be cleaved by caspase-3. May recognize additional bands, possibly corresponding to HAUSP cleavage products. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Highly Toxic
      ReferencesLi, M., et al. 2004. Mol. Cell 13, 879.
      Muyang, L., et al. 2002. Nature 416, 648.
      Vugmeyster, Y., et al. 2002. Mol. Immunol. 39, 431.