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MABN702 Anti-HK2 Antibody, clone 3D3

Detect Hexokinase II using this mouse monoclonal antibody, Anti-HK2 Antibody, clone 3D3 validated for use in western blotting, IHC & Flow Cytometry.

Anti-HK2 Antibody, clone 3D3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: HKII, HXK2, DKFZp686M1669, HK2 UniProt Number: P52789
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MABN702
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, FCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN702
      DescriptionAnti-HK2 Antibody, clone 3D3
      Alternate Names
      • Hexokinase-2
      • Hexokinase type II
      • HK II
      • Muscle form hexokinase
      • HK2
      Background InformationThere are four hexokinases in most vertebrates, hexokinase I, II, III, and IV; HK2 is hexokinase-II (Hexokinase-2). Hexokinase-2 (HK2) or alternatively Hexokinase type II (HK II) or muscle form hexokinase is the predominant hexokinase enzyme expressed in insulin-responsive tissues like skeletal muscle and other tissues like brain. HK2 is also expressed at high levels in many cancers which is thought to aid in their survival. HK2 is responsible for the phosphorylation of hexoses such as glucose into glucose-6-phosphate; the initial step in carbohydrate metabolism. HK2 is typically associated with mitochondrial membranes. Over expression of HK2 is associated with resistance to cell death and often given the name of the “Warburg Effect” after the initial discover of the relationship between energy metabolism and cell death. While HK2 overexpression has been shown to be one of the mechanisms for the survival of cancers, it has also been shown to facilitate the survival of normal, non-cancerous tissues such as neurons and astrocytes in response to injury and stress. Thus because of its relationship to energy metabolism and its connection with cell death and survival, HK2 is being examined as a potential target for treatment of everything from brain tumors to aging. EMD-Millipore’s Anti-HK2 mouse monoclonal has been tested in western blot on Jurkat, HeLa, and HEK293 cell lysates and in paraffin embedded immunohistochemistry on esophagus and human lung cancer tissues and in flow cytometry on K562 cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • Jurkat, HeLa, and HEK293 cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Hexokinase II using this mouse monoclonal antibody, Anti-HK2 Antibody, clone 3D3 validated for use in western blotting, IHC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected HK2 in human esophagus cancer and lung cancer tissues.

      Flow cytometry analysis: A 1:200-400 dilution from a representative lot detected HK2 in K562 cells.

      Optimal working dilutions must be determined by end user.


      Biological Information
      ImmunogenPurified recombinant fragment of human HK2 expressed in E. Coli.
      Clone3D3
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • HKII
      • HXK2
      • DKFZp686M1669
      • HK2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~102 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat, HeLa, and HEK293 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected HK2 in Jurkat, HeLa, and HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN702 04053252918537

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies