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MABF3178-25UG Anti-IL-22 Antibody, clone 9F2.2.4

Anti-IL-22, clone 9F2.2.4, Cat. No. MABF3178, is a mouse monoclonal antibody that detects human IL-22 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABF3178-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3178-25UG
      DescriptionAnti-IL-22 Antibody, clone 9F2.2.4
      Alternate Names
      • Interleukin-22
      • Cytokine Zcyto18
      • IL-10-related T-cell-derived-inducible factor
      • IL-TIF
      Background InformationInterleukin-22 (UniProt: Q9GZX6; also known as IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF) is encoded by the IL22 (also known as ILTIF, ZCYTO18, UNQ3099/PRO10096) gene (Gene ID: 50616) in human. IL-22 is an -helical cytokine that contributes to the inflammatory response in vivo. It is a secretory protein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to produce the mature protein. It is immediately produced to initiate immune response against several tissue impairments. It is considered as a fundamental mediator in inflammation, mucous production, protective role against pathogens, wound healing, and tissue regeneration. It is produced by several populations of immune cells, including macrophages, β T cells classes Th1, Th22, and Th17 cells at the site of inflammation. It is shown to up-regulate a number of mucus associated genes, such as MUC1, MUC3, MUC10, and MUC13 in colonic and tracheal epithelial cells. Along with IL-17A, it also produces pulmonary mucus as a control against Gram negative pathogens. Its biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein (IL-22BP). IL-22 is shown to have a very high affinity for binding to IL-22BP, which acts as a natural antagonist of IL-22. IL-22BP binding occurs at specific sites on IL-22 that are also utilized by IL-22R1. (Ref.: Arshad, T., et al. (2020). Front. Immunol. 11; 2148).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-22, clone 9F2.2.4, Cat. No. MABF3178, is a mouse monoclonal antibody that detects human IL-22 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-22 in human tonsil tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA full-length mature human IL-22 (without the signal peptide).
      EpitopeUnknown.
      Clone9F2.2.4
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 9F2.2.4 is a mouse monoclonal antibody that detects human IL-22.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL22
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~17 kDa observed; 20.01 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human IL-22.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant human IL-22.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3178-25UG 04065267403953