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MAC140-25UG Anti-Inntag™ 10 (IT10) Antibody, clone IT10

Anti-Inntag™ 10 (IT10), clone IT10 Antibody, Cat. No. MAC140-25UG, validated for use in Western Blotting and Immunocytochemistry.

Research Category: Epigenetics & Nuclear Function Gene Symbol: N/A UniProt Number: P10414
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MAC140-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAC140-25UG
      DescriptionAnti-Inntag™ 10 (IT10) Antibody, clone IT10
      Alternate Names
      • Allergen Amb t V
      • Allergen Ra5G
      • Amb t 5
      Background InformationPollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag ™ IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag ™ IT10 is based on a packed beta-sheet conformation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Inntag™ 10 (IT10), clone IT10 Antibody, Cat. No. MAC140-25UG, validated for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Inntag ™ 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunocytochemistry Analysis: A representative lot detected Inntag ™ 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
      Biological Information
      ImmunogenHis-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.
      IsotypeIgG1
      Species Reactivity
      • All
      Species Reactivity NoteAmbrosia trifida.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N/A
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAC140-25UG 04054839421020

      Documentation