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MABN2274-25UL Anti-LMO4 Antibody, clone 20F8

Anti-LMO4, clone 20F8, Cat. No. MABN2274, is a highly specific rat monoclonal antibody that targets LIM domain transcription factor LMO4 and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

MABN2274-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2274-25UL
      DescriptionAnti-LMO4 Antibody, clone 20F8
      Alternate Names
      • LIM domain transcription factor LMO4
      • Breast tumor autoantigen
      • LIM domain only protein 4
      Background InformationLIM domain transcription factor LMO4 (UniProt: P61969; also known as Breast tumor autoantigen, LIM domain only protein 4, LMO-4) is encoded by the Lmo4 gene (Gene ID: 16911) in murine species. LMO4 is a member of the LIM-only (LMO) family of transcription regulators that are known to serve as molecular adaptors and provide a scaffold for multiprotein complexes of DNA-binding factors and transcriptional regulatory proteins. It contains two zinc binding domains (aa 23-83 and 87-147). LMO4 is widely expressed and plays a role in embryonic development. It is shown to be over-expressed in breast carcinomas and act as a negative regulator of mammary epithelial differentiation. Overexpression of LMO4 induces mammary hyperplasia and promotes cell invasion. It is a good predictor of poor outcome in breast cancer. Mammary glands that are deficient in LMO4 display impaired lobuloalveolar development. (Ref.: Murphy, NC et al., (2008). Br. J. Cancer 98(3); 537-541; Sum, EYM et al., (2005). Oncogene 24(30); 4820 -4828).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-LMO4, clone 20F8, Cat. No. MABN2274, is a highly specific rat monoclonal antibody that targets LIM domain transcription factor LMO4 and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated LMO4 in Immunoprecipitation applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86).

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected LMO4 in Immunohistochemistry applications (Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Murphy, N.C., et. al. (2008). Br J Cancer. 98(3):537-41; Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).

      Western Blotting Analysis: A representative lot detected LMO4 in Western Blotting applications (Sum, E.Y., et. al. (2005). Oncogene. 24(30):4820-8; Sum, E.Y., et. al. (2005). J Histochem Cytochem. 53(4):475-86; Sum, E.Y., et. al. (2005). Proc Natl Acad Sci U S A. 102(21):7659-64).
      Biological Information
      ImmunogenGST-tagged full-length mouse LIM domain transcription factor LMO4.
      Clone20F8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 20F8 is a rat monoclonal antibody that detects LIM domain transcription factor LMO4 in murine species.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Bovine
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Bovine and Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Lmo4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~18 kDa observed; 17.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected LMO4 in MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2274-25UL 04054839646829