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ABE484-25UG Anti-LOXL2

nti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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ABE484-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE484-25UG
      DescriptionAnti-LOXL2
      Alternate Names
      • Lysyl oxidase homolog 2
      • EC: 1.4.3.13
      • Lysyl oxidase-like protein 2
      • Lysyl oxidase-related protein 2
      • Lysyl oxidase-related protein WS9-14
      Background InformationLysyl oxidase homolog 2 (UniProt: Q9Y4K0; also known as EC: 1.4.3.13, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 LOXL2) is encoded by the LOXL2 gene (Gene ID: 4017) in human. LOXL2 localizes to both cytoplasmic and nuclear region. It catalyzes the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, it promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOXL2 also acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When present in the nucleus, it acts as a transcription corepressor and specifically mediates deamination of trimethylated Lysine 4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Overexpression of LOXL2 has been reported in a number of cancers and its ability to promote epithelial to mesenchymal transition suggest that it might play a role in tumor progression. Higher expression has been correlated with metastasis and reduced survival in patients with aggressive breast cancer. LOXL2 activity is strongly induced in hypoxia and it is reported to be a direct transcriptional target of HIF1A.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Applicationnti-LOXL2, Cat. No. ABE484, is a highly specific rabbit polyclonal antibody that targets Lysyl oxidase homolog 2 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected LOXL2 in MDA-MB-231 cell lysate.
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LOXL2 in human prostate and human placenta tissues.
      Immunoprecipitation Analysis: A representative lot detected LOXL2 in LoxL2 expressing vector (Courtesy Dr Sandra Peiro from the  Institut Hospital del Mar d'Investigacions Mèdiques (IMIM) in Barcelona Spain ).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human Lysyl oxidase homolog 2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Lysyl oxidase homolog 2 in human cells. It targets an epitope within 16 amino acids from the N-terminal half.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • LOXL2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~82 kDa obseerved; 86.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected LOXL2 in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE484-25UG 04054839526879