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AB2290 Anti-MAP-2 Antibody

Anti-MAP-2 Antibody, Cat. No. AB2290, is a highly specific rabbit polyclonal antibody that targets rat MAP-2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-MAP-2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers Gene Symbol: MAP2, MAP2B, MAP-2, MAP2C, MAP2A UniProt Number: P15146
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AB2290
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWB, IH(P), ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB2290
      DescriptionAnti-MAP-2 Antibody
      Alternate Names
      • microtubule-associated protein 2
      • MAP2
      • MAP 2
      Background InformationMicrotubule-associated protein 2 (UniProt: P15146; also known as MAP-2) is encoded by the Map2 (also known as Mtap2) gene (Gene ID: 25595) in rat. MAP-2 belong to the family of thermostable proteins associated with microtubules. It is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules against depolymerization. It also has a stiffening effect on microtubules. MAP-2 is shown to be essential for the development and maintenance of neuronal morphology. Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene) have been identified in neurons. The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation. All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif. MAP-2 can be phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that causes its detachment from microtubules and results in their disassembly.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat brain microsomal tissue lysate
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MAP-2 Antibody, Cat. No. AB2290, is a highly specific rabbit polyclonal antibody that targets rat MAP-2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in E18 rat hippocampus neurons.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in mouse cerebellum, rat cerebral cortex, and rat kidney tissues.
      Biological Information
      ImmunogenThree KLH-conjugated linear peptides corresponding to the N-terminal and central regions of rat MAP-2 .
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes MAP-2.
      Species Reactivity
      • Rat
      Species Reactivity NoteDemonstrated to react with rat.
      Other homologies: Mouse (94% sequence homology). Human (72% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRegulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities [RGD].
      Gene Symbol
      • MAP2
      • MAP2B
      • MAP-2
      • MAP2C
      • MAP2A
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~270 kDa observed; 202.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain microsomal preparation.

      Western Blotting Analysis: 1 µg/mL of this antibody detected MAP-2 in 10 µg of rat brain microsomal preparation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB2290 04053252414527

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies