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MABF2044 Anti-MCPyV large T-antigen Antibody, clone CM2B4

Anti-MCPyV large T-antigen, clone CM2B4, Cat. No. MABF2044, is a mouse monoclonal antibody that detects MCPyV large T antigen and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting for the detection of Merkel cell polyomavirus.

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Research Category: Inflammation & Immunology Gene Symbol: N/A
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MABF2044
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2044
      DescriptionAnti-MCPyV large T-antigen Antibody, clone CM2B4
      Alternate Names
      • Large T antigen
      • MCV Large T antigen
      Background InformationMerkel cells are neuroendocrine cells found in skin that have synaptic contacts with somatosensory afferents. These cells can turn malignant and form an aggressive form of skin cancer, which is known as Merkel cell carcinoma (MCC). A vast majority of MCC are caused by a polyomavirus known as Merkel cell polyomavirus (MCPyV or MCV). The MCPyV genome is reported to undergo clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen. Full-length MCPyV large T-antigen is a 125 kDa nuclear protein, however, MCPyV T-antigens obtained from MCC have natural truncating mutations, which result in variably-sized, smaller proteins. Based on homology to other polyomaviruses, the MCPyV large and small T antigens are predicted to be oncogenic and contribute directly to the carcinogenesis of MCC. MCPyV large T antigen can serve as a specific marker for MCC.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MCPyV large T-antigen, clone CM2B4, Cat. No. MABF2044, is a mouse monoclonal antibody that detects MCPyV large T antigen and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting for the detection of Merkel cell polyomavirus.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected MCPyV large T-antigen in Western Blotting applications (Liu, X., et. al. (2011). J Biol Chem. 286(19):17079-90; Rodig, S.J., et. al. (2012) J Clin Invest. 122(12):4645-53).

      Immunoprecipitation Analysis: A representative lot detected MCPyV large T-antigen in Immunoprecipitation applications (Liu, X., et. al. (2011). J Biol Chem. 286(19):17079-90).

      Immunohistochemistry Analysis: A representative lot detected MCPyV large T-antigen in Immunohistochemistry applications (Rodig, S.J., et. al. (2012) J Clin Invest. 122(12):4645-53; Busam, K.J., et. al. (2009). Am J Surg Pathol. 33(9):1378-85).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to RSRKPSSNASRGA sequence from the Merkel cell polyomavirus large T antigen exon 2 with a C-terminal cystiene.
      CloneCM2B4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone CM2B4 is highly specific for MCPyV large T and 57kT isoforms, but does not detect MCPyV small T antigen.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N/A
      Purification MethodProtein G purified
      Molecular Weight45 kDa observed for truncaed form. For intact form MW 125 kDaUncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MKL-1 cell lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected MCPyV large T-antigen in 10 µg of MKL-1 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2044 04054839328749

      Documentation

      Anti-MCPyV large T-antigen Antibody, clone CM2B4 Certificates of Analysis

      TitleLot Number
      Anti-MCPyV large T-antigen, clone CM2B4 - 3190907 3190907
      Anti-MCPyV large T-antigen, clone CM2B4 - 3689465 3689465
      Anti-MCPyV large T-antigen, clone CM2B4 - 3821564 3821564
      Anti-MCPyV large T-antigen, clone CM2B4 - 3906513 3906513
      Anti-MCPyV large T-antigen, clone CM2B4 - 4028622 4028622
      Anti-MCPyV large T-antigen, clone CM2B4 - 4082558 4082558
      Anti-MCPyV large T-antigen, clone CM2B4 - 4231239 4231239
      Anti-MCPyV large T-antigen, clone CM2B4 - 4320732 4320732
      Anti-MCPyV large T-antigen, clone CM2B4 Monoclonal Antibody Q2906696
      Anti-MCPyV large T-antigen, clone CM2B4 Monoclonal Antibody 3090205