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CB1020 Anti-MFN2 Mouse mAb (4H8)

Anti-MFN2 Mouse mAb (4H8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Mitofusin 2

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~75 kDa MFN2 protein in HeLa and PC-12 cells and human heart tissue.
      Catalogue NumberCB1020
      Brand Family Calbiochem®
      SynonymsAnti-Mitofusin 2
      Application Data
      Detection of human MNF2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-MFN2 Mouse mAb (4H8) (Cat. No. CB1020) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of rat MNF2 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-MFN2 Mouse mAb (4H8) (Cat. No. CB1020) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MNF2 by staining paraffin seciotns. Sample: Formalin-fixed paraffin-embedded human heart tissue. Primary antibody: Anti-MFN2 Mouse mAb (4H8) (Cat. No. CB1020) (1-5 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesMoran, M., et al. 2010. Biochim Biophys. Acta. 1802 443.
      Holloway, G,P., et al. 2008. Am. J. Physiol. Endocrinol. Metab. 294 E1060.
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, PC-12 cells, Human heart tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 661-758 of human MFN2, expressed as a GST fusion protein
      ImmunogenNA
      Clone4H8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1020 0

      Documentation

      Anti-MFN2 Mouse mAb (4H8) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Moran, M., et al. 2010. Biochim Biophys. Acta. 1802 443.
      Holloway, G,P., et al. 2008. Am. J. Physiol. Endocrinol. Metab. 294 E1060.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-Mitofusin 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (5 µg/ml)
      Application Data
      Detection of human MNF2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-MFN2 Mouse mAb (4H8) (Cat. No. CB1020) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of rat MNF2 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-MFN2 Mouse mAb (4H8) (Cat. No. CB1020) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MNF2 by staining paraffin seciotns. Sample: Formalin-fixed paraffin-embedded human heart tissue. Primary antibody: Anti-MFN2 Mouse mAb (4H8) (Cat. No. CB1020) (1-5 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa MFN2 protein.
      BackgroundMFN2 is a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity.
      HostMouse
      Immunogen speciesNA
      ImmunogenA recombinant polypeptide corresponding to amino acids 661-758 of human MFN2, expressed as a GST fusion protein
      Clone4H8
      IsotypeIgG2a
      Specieshuman, rat
      Positive controlHeLa cells, PC-12 cells, Human heart tissue
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesMoran, M., et al. 2010. Biochim Biophys. Acta. 1802 443.
      Holloway, G,P., et al. 2008. Am. J. Physiol. Endocrinol. Metab. 294 E1060.