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AM50 Anti-Mcl-1 (Ab-1) Mouse mAb (RC13)

Anti-Mcl-1 (Ab-1) Mouse mAb (RC13) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~42 kDa Mcl-1 protein in ML1 cells and spleen and tonsil tissue.
      Catalogue NumberAM50
      Brand Family Calbiochem®
      Application Data
      Detection of human Mcl-1 by immunoblotting. Sample: Human Mcl-1. Primary antibody: Anti-Mcl-1 (Ab-1) Mouse mAb (RC13) (Cat. No. AM50) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesZhou, P., et al. 1997. Blood 89, 630.
      McDonnell, T.J., et al. 1996. Experientia 52, 1008.
      Yang, T., et al. 1996. J. Cell. Physiol. 166, 523.
      Craig, R.W. 1995. Semin. Cancer Biol. 6, 35.
      Krajewski, S., et al. 1995. Am. J. Pathol. 146, 1309.
      Ohta, K., et al., 1995. Int. Immunol. 7, 1817.
      Sedlak, T.W., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 7834.
      Yang, T., et al. 1995. J. Cell Biol. 128, 1173.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlML-1 cells or spleen or tonsil tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:200-1:400)
      Immunoprecipitation (10 µl/mg protein lysate)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-327 of human Mcl-1
      ImmunogenHuman
      CloneRC13
      HostMouse
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM50 0

      Documentation

      Anti-Mcl-1 (Ab-1) Mouse mAb (RC13) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Zhou, P., et al. 1997. Blood 89, 630.
      McDonnell, T.J., et al. 1996. Experientia 52, 1008.
      Yang, T., et al. 1996. J. Cell. Physiol. 166, 523.
      Craig, R.W. 1995. Semin. Cancer Biol. 6, 35.
      Krajewski, S., et al. 1995. Am. J. Pathol. 146, 1309.
      Ohta, K., et al., 1995. Int. Immunol. 7, 1817.
      Sedlak, T.W., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 7834.
      Yang, T., et al. 1995. J. Cell Biol. 128, 1173.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      ApplicationImmunoblotting (1:200-1:400)
      Immunoprecipitation (10 µl/mg protein lysate)
      Application Data
      Detection of human Mcl-1 by immunoblotting. Sample: Human Mcl-1. Primary antibody: Anti-Mcl-1 (Ab-1) Mouse mAb (RC13) (Cat. No. AM50) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with p3-NS-1/Ag4-1 mouse myeloma cells. Recognizes the ~42 kDa Mcl-1 protein.
      BackgroundMcl-1 also known as myeloid cell leukemia-1 was originally identified as an early gene expressed in ML-1 cells during phorbol ester induced differentiation along the monocyte/macrophage pathway. Mcl-1 is a member of the bcl-2 family of apoptosis markers and is the anti-apoptotic homologue of bcl-2. The C-terminal regions of Mcl-1 and bcl-2 share significant sequence homology. Mcl-1 together with bcl-2 and the pro-apoptotic protein, bax are thought to function by forming homo- and heterotypic dimers that are critical in controlling the apoptotic process. Like bcl-2, Mcl-1 can be found in epithelial cells in a variety of tissues including prostate, breast, endometrium, epidermis, stomach, intestine colon and respiratory tract although with different expression patterns. Interestingly, in contrast to bcl-2, little or no Mcl-1 is detected in neurons of the brain and spinal cord however, Mcl-1 is highly expressed in cardiac and skeletal muscle which contain relatively less bcl-2. Chondrocytes and hepatocytes do not express bcl-2 but do express Mcl-1. Expression of Mcl-1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g., ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and bax and a decrease in bcl-2. Enhanced expression of Mcl-1 prominently associated with mitochondria complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, Mcl-1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 1-327 of human Mcl-1
      CloneRC13
      IsotypeIgG
      Specieshuman
      Positive controlML-1 cells or spleen or tonsil tissue
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesZhou, P., et al. 1997. Blood 89, 630.
      McDonnell, T.J., et al. 1996. Experientia 52, 1008.
      Yang, T., et al. 1996. J. Cell. Physiol. 166, 523.
      Craig, R.W. 1995. Semin. Cancer Biol. 6, 35.
      Krajewski, S., et al. 1995. Am. J. Pathol. 146, 1309.
      Ohta, K., et al., 1995. Int. Immunol. 7, 1817.
      Sedlak, T.W., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 7834.
      Yang, T., et al. 1995. J. Cell Biol. 128, 1173.