Millipore Sigma Vibrant Logo

ABN1728 Anti-MeCP2

Anti-MeCP2 Antibody, Cat. No. ABN1728, is a highly specific rabbit polyclonal antibody that targets MeCP2 and has been tested in Immunofluorescence and Western Blotting.

Anti-MeCP2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: MECP2, AS, MRXS13, MRXSL, PPMX, RTT, AUTSX3 UniProt Number: P51608
View Products on Sigmaaldrich.com
ABN1728
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1728
      DescriptionAnti-MeCP2
      Alternate Names
      • Methyl-CpG-binding protein 2
      • MeCp-2 protein
      • MeCp2
      Background InformationMethyl-CpG-binding protein 2 (UniProt P51608; also known as MeCp-2 protein, MeCp2) is encoded by the MECP2 (also known as AS, MRXS13, MRXSL, PPMX, RTT, AUTSX3) gene (Gene ID 4204) in human. Methyl-CpG-binding proteins that are involved in gene expression regulation by targeting methylated DNA residues in gene promoters via a conserved methyl-CpG-binding domain (MBD; a.a. 90-162 of human MeCp2). Upon binding methylated CpG sites on the DNA strands, transcriptional repression domain (TRD; a.a. 207-492) then mediates association with SIN3A to recruit histone deacetylases (HDAC). The TRD region also contains a nuclear localization signal (NLS) sequence (a.a. 255-271) and a nuclear receptor co-repressor 2/NCoR2-interacting site (a.a. 269-309). MeCp2 protein is found in all cells in the body, including the brain, where it is found in high concentrations in neurons and is associated with maturation of the central nervous system (CNS) and in forming synaptic contacts. In contrast to other MBD family members, MECP2 is X-linked and subject to X chromosome inactivation. MECP2 gene mutations are linked to Rett syndrome (RTT), a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.03% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-MeCP2 Antibody, Cat. No. ABN1728, is a highly specific rabbit polyclonal antibody that targets MeCP2 and has been tested in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1:1000 dilution from a representative lot detected MeCp2 in mouse brain tissue sections (Courtesy of EnCor Biotechnology Inc.).
      Biological Information
      ImmunogenLinear peptide corresponding to human MeCp2 C-terminal end sequence.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets MeCp2 C-terminal end sequence conserved between spliced isoform A (isoform Beta) and isoform B (isoform Alpha).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteMouse, Human. Predicted to react with Rhesus Macaque, Bovine, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MECP2
      • AS
      • MRXS13
      • MRXSL
      • PPMX
      • RTT
      • AUTSX3
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~70 kDa observed. 52.44/52.31 kDa (human/mouse isoform A or Beta) and 53.32/53.58 kDa (human/mouse isoform B or Alpha) calculated. The larger-than-predicted band size is consistent with that reported in the literature (Jarrar, M.H., et al. (2003). J. Child Neurol. 18(10):675-682). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain nuclear extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected MeCp2 in 10 µg of mouse brain nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1728 04054839055096