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MABN1728 Anti-Navbeta2 Antibody, clone N395/68

Detect Navbeta2 using this Anti-Navbeta2 Antibody, clone N395/68 validated for use in Western Blotting, Immunohistochemistry.

Anti-Navbeta2 Antibody, clone N395/68 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: Scn2b, Gm183, AI840361 UniProt Number: Q56A07
MABN1728
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1728
      DescriptionAnti-Navbeta2 Antibody, clone N395/68
      Alternate Names
      • Sodium channel subunit beta-2
      • Navbeta2
      • Sodium channel, voltage-gated, type II, beta polypeptide
      Background InformationSodium channel subunit beta-2 (UniProt Q56A07; also known as Navbeta2, Sodium channel, voltage-gated, type II, beta polypeptide) is encoded by the Scn2b (also known as Gm183, AI840361, 2810451E09Rik) gene (Gene ID 72821) in murine species. Sodium channels are integral plasma membrane proteins that conduct Na+ transport across the plasma membrane. They are classified as either voltage-gated or ligand-gated based on the nature of the triggering mechanism for channel activation. Voltage-gated sodium channels (VGSCs) are also called voltage-sensitive, voltage-dependent, or Nav channels. VGSCs normally consist of an alpha subunit that forms the ion conducting pore and one to two beta subunits that have several functions including channel gating modulation, although the expression of the alpha subunit alone is sufficient to produce a functional channel. There exist nine known alpha subunits (Nav1.1 through Nav1.5 encoded by SCN1A through SCN5A and Nav1.6 through Nav1.9 encoded by SCN8A through SCN11A) and four beta subunits (Navbeta1 through Navbeta4 encoded by SCN1B through SCN4B). Beta 1 and beta 3 interact with the alpha subunit non-covalently, whereas beta 2 and beta 4 associate with alpha via disulfide bond. Beta subunits are members of the Ig superfamily and contain a prototypic V-set Ig loop in their extracellular domain. Beta subunits share no homology with their counterparts of calcium and potassium channels, instead are homologous to neural cell adhesion molecules (CAMs) and the family of L1 CAMs. Navbeta2 is initially produced with a signal peptide sequence (a.a. 1-29), the remmoval of which yields the mature protein with an Ig-like C2-type extracellular domain (a.a. 30-159), a transmembrane segment (a.a. 160-180), and a small cytoplamic tail (a.a. 181-215).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Navbeta2 using this Anti-Navbeta2 Antibody, clone N395/68 validated for use in Western Blotting, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected Navbeta2 in 10 µg of rat brain tissue lysate.
      Immunohistochemistry Analysis: A representative lot detected Navbeta2 immunoreactivity in adult rat hippocampus sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
      Western Blotting Analysis: A representative lot detected Navbeta2 only in brain lysates from wild-type rats and mice, but not in brain lysates from Scn2b-knockout mice.
      Biological Information
      ImmunogenRecombinant full-length mouse Navbeta2.
      CloneN395/68
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone N395/68 detects the sodium channel subunit beta-2 (Navbeta2) target band only in brain lysates from wild-type rats and mice, but not brain lysates from Scn2b-knockout mice. Clone N395/68 does not cross-reacts with Navbeta1, Navbeta3 or Navbeta4.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Scn2b
      • Gm183
      • AI840361
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~38 kDa observed. Target band size appears larger than the calculated molecular weight of 24.23 kDa due to glycosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Navbeta2 in mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1728 04055977315806