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MABC1722-25UG Anti-PTEN/PTEN-Long Antibody, clone 1A1

Anti-PTEN/PTEN-Long, clone 1A1, Cat. No. MABC1722, is a mouse monoclonal antibody that detects PTEN and PTEN-Long and is tested for use in ELISA.

MABC1722-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1722-25UG
      DescriptionAnti-PTEN/PTEN-Long Antibody, clone 1A1
      Alternate Names
      • Phosphatidylinositol 3
      • 4
      • 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
      • EC:3.1.3.16
      • Mutated in multiple advanced cancers 1
      • Phosphatase and tensin homolog
      Background InformationPhosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (UniProt: P60484; also known as Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN) is encoded by the PTEN (also known as MMAC1, TEP1) gene (Gene ID: 5728) in human. PTEN belongs to the family of lipid phosphatases. It contains an N-terminal phosphatase catalytic domain, a C2-domain that binds to phospholipid membranes, and a C-terminal regulatory tail. It is a dual-specific phosphatase, which dephosphorylates both lipid and protein substrates. PTEN blocks the phosphatidylinositol 3-kinase/Akt signaling pathway by removing the phosphate in the 3-position on phosphoinositide-3, 4, 5-trisphosphate (PIP3). Dephosphorylation of PIP3 is an important factor in cell growth, proliferation, apoptosis, and survival. Dephosphorylation of PIP3 blocks Akt signaling that can lead to higher activity of pro-apoptotic molecules such as Bad and Caspase-9. PTEN activity, stability, localization, and conformation are controlled through complex regulatory mechanisms, including the reversible phosphorylations of several Serine/Threonine residues in its unfolded C-terminal tail region (PTEN-C-tail, residues 351-403). Alternative translation of PTEN is reported to encode a 576-amino acid translational variant that is known as PTEN-Long with a 173-amino acid domain at its N-terminus followed by the classical 403 amino acids of PTEN. PTEN-Long is a membrane permeable, secreted protein that can enter other cells and acts as an antagonist of the PI3K pathway. PTEN is considered a major tumor suppressor and its mutations are reported in several human tumors. Over seventy different types of PTEN mutation have been recorded in patients with Cowden syndrome. PTEN -/- mice exhibit higher levels of 3 -phosphorylated phospholipids and die during embryogenesis due to the failure of developmental apoptosis. Clone 1A1 captures both PTEN and PTEN-Long and can be used both as capture and detection antibody. (Ref.: Hopkins, BD., et al. (2013). Science. 341(6144); 399-402).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PTEN/PTEN-Long, clone 1A1, Cat. No. MABC1722, is a mouse monoclonal antibody that detects PTEN and PTEN-Long and is tested for use in ELISA.
      Key Applications
      • ELISA
      Biological Information
      ImmunogenA bacterial fusion protein with a V5/His-tag at its C- terminus.
      EpitopeC-terminal
      Clone1A1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1A1 is a mouse monoclonal antibody that detects human PTEN. It can target both PTEN and PTEN-Long.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTEN
      • MMAC1
      • TEP1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight47.17 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with PTEN fusion protein.

      ELISA Analysis (ELISA): Various dilution (starting at 5 mg/mL, two-fold serial dilutions; 6 points) of this antibody detected PTEN fusion protein. Anti-PTEN-Long, clone 1C2 (MABS450B) was used as a detection antibody.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1722-25UG 04061841879848