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MABN730 Anti-Rab10 Antibody, clone 4E2

Detect Ras related protein Rab-10 using this mouse monoclonal antibody, Anti-Rab10 Antibody, clone 4E2 validated for use in western blotting & Immunofluorescence.

Anti-Rab10 Antibody, clone 4E2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: RAB10
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MABN730
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IFMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN730
      DescriptionAnti-Rab10 Antibody, clone 4E2
      Alternate Names
      • RAB10
      • Ras-related protein Rab-10
      • Rab10
      Background InformationRas related protein Rab-10 (Rab10) is encoded by the gene named RAB10 and is member of the small GTPase family of Rab proteins. Rab proteins play critical roles in intracellular trafficking from the formation of vesicles to their fusion with membranes. Specifically Rab10 is involved in regulating RME-1 in endocytic recycling as well as endoplasmic reticulum structural dynamics and morphology, and Rab10 also appears to be critical to formation and correct placement of proteins in such critical cellular structures as the basement membranes of during organ morphogenesis and basement membrane remodeling during development. Rab10 localizes to internal membrane structures like the endoplasmic reticulum and Golgi apparatus but in certain cells it can be found associated with cilia and other cellular structures as well. EMD-Millipore’s Anti-Rab10 mouse monoclonal antibody has been tested in western blot against human HeLa and mouse NIH3T3 cell lysates and in fluorescent immunocytochemistry on LOVO cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • HeLa and NIH/3T3 cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Ras related protein Rab-10 using this mouse monoclonal antibody, Anti-Rab10 Antibody, clone 4E2 validated for use in western blotting & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Rab10 in LOVO cells.

      Optimal working dilutions must be determined by end user.



      Biological Information
      ImmunogenPurified recombinant fragment of human Rab10 expressed in E. Coli.
      Clone4E2
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • RAB10
      Purification MethodUnpurified
      Molecular Weight~25 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa and NIH/3T3 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected Rab10 in HeLa and NIH/3T3 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN730 04053252918575

      Documentation

      Anti-Rab10 Antibody, clone 4E2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Rab10 Antibody, clone 4E2 Certificates of Analysis

      TitleLot Number
      Anti-Rab10, clone 4E2 - QVP1305213 QVP1305213
      Anti-Rab10, clone 4E2 - VP1805042 VP1805042
      Anti-Rab10, clone 4E2 - VP1905242 VP1905242
      Anti-Rab10, clone 4E2 - VP2301050 VP2301050
      Anti-Rab10, clone 4E2 - VP2401315 VP2401315
      Anti-Rab10, clone 4E2 - VP2401316 VP2401316
      Anti-Rab10, clone 4E2 -VP1504074 VP1504074
      Anti-Rab10, clone 4E2 -VP1602116 VP1602116
      Anti-Rab10, clone 4E2 -VP1605049 VP1605049
      Anti-Rab10, clone 4E2 -VP1611250 VP1611250
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