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OP28 Anti-Rb (Ab-1) Mouse mAb (C36)

Anti-Rb (Ab-1) Mouse mAb (C36) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Retinoblastoma Associated Protein, Anti-pp105

OP28
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~105 kDa Rb protein in ML-1 cells. Does not cross-react wtih the Rb-related proteins p107 or p130. Sold under license of U.S. Patent 5,853,988 and corresponding patents.
      Catalogue NumberOP28
      Brand Family Calbiochem®
      SynonymsAnti-Retinoblastoma Associated Protein, Anti-pp105
      References
      ReferencesHorowitz, J.M., et al. 1989. Science 243, 937.
      Whyte, P., et al. 1988. Nature 334, 124.
      Klein, G., 1987. Science 238, 1539.
      Lee, W.H., et al. 1987. Science 235, 1394.
      Lee, W.H., et al. 1987. Nature 329, 642.
      Knudson, A.G., 1971. Proc. Natl. Acad. Sci. 68, 8200.
      Product Information
      DeclarationSold under license of U.S. Patent 5,853,988 and corresponding patents.
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlY79 or Saos-2 cells
      Positive controlML-1 cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesEpitope Identification Whyte, P., et al. 1988. Nature 334, 124.
      Key Applications Immunoprecipitation
      Application NotesImmunoprecipitation (1 µg antibody or use Cat. No. OP28A)
      Application CommentsDoes not cross-react wtih the Rb-related proteins p107 or p130. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogencellular Rb
      Epitopewithin amino acids 300-380
      CloneC36
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP28 0

      Documentation

      Anti-Rb (Ab-1) Mouse mAb (C36) Certificates of Analysis

      TitleLot Number
      OP28

      References

      Reference overview
      Horowitz, J.M., et al. 1989. Science 243, 937.
      Whyte, P., et al. 1988. Nature 334, 124.
      Klein, G., 1987. Science 238, 1539.
      Lee, W.H., et al. 1987. Science 235, 1394.
      Lee, W.H., et al. 1987. Nature 329, 642.
      Knudson, A.G., 1971. Proc. Natl. Acad. Sci. 68, 8200.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-October-2007 RFH
      SynonymsAnti-Retinoblastoma Associated Protein, Anti-pp105
      ApplicationImmunoprecipitation (1 µg antibody or use Cat. No. OP28A)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~105 kDa Rb protein.
      BackgroundCertain genes have been identified whose function appears to be the suppression of tumor formation. Once such gene has been linked to the hereditary cancer retinoblastoma. The retinoblastoma (Rb) gene encodes a protein of 928 amino acids which is found predominately in the nucleus. While all the biochemical functions of the protein are not known, it has the capability of binding DNA. The Rb gene is found in the DNA of quail, mice, rats and monkeys. The Rb protein may be modified by phosphorylation. While there are several potential sites for N-linked glycosylation, no modification has been detected. The Rb protein has been reported as having apparent molecular weights of ~105 kDa, ~100-114 kDa and ~102 or ~104 kDa which are antigenically related. The Rb protein has been detected in at least 20 different common laboratory cell lines with the exception of retinoblastoma cells. Malignant transformation of cells is caused by inactivation of the gene by mutation but also by inactivation of the Rb protein by binding to the adenovirus E1A protein or SV-40 T antigen. This has lead to the classification of the Rb gene as a tumor suppressor gene.
      HostMouse
      Immunogencellular Rb
      Epitopewithin amino acids 300-380
      CloneC36
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlML-1 cells
      Negative controlY79 or Saos-2 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react wtih the Rb-related proteins p107 or p130. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesHorowitz, J.M., et al. 1989. Science 243, 937.
      Whyte, P., et al. 1988. Nature 334, 124.
      Klein, G., 1987. Science 238, 1539.
      Lee, W.H., et al. 1987. Science 235, 1394.
      Lee, W.H., et al. 1987. Nature 329, 642.
      Knudson, A.G., 1971. Proc. Natl. Acad. Sci. 68, 8200.
      Application referencesEpitope Identification Whyte, P., et al. 1988. Nature 334, 124.