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ST1122 Anti-Rictor Mouse mAb (2C8)

Anti-Rictor Mouse mAb (2C8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Rapamycin-Insensitive Companion of mTOR Mouse mAb

ST1122
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~190 kDa Rictor protein in HEK293 cells.
      Catalogue NumberST1122
      Brand Family Calbiochem®
      SynonymsAnti-Rapamycin-Insensitive Companion of mTOR Mouse mAb
      Application Data
      Lane 1: Detection of human Rictor by immunoblotting. Sample: Whole cell lysate (40 µg) from HEK293 cells. Primary antibody: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (1 µg/ml). Detection: chemiluminescence. Lanes 2 and 3: Detection of mTOR/Rictor by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Antibody for IP: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (10 µg/mg total protein). Immunoblotting conditions: Samples: half of Rictor IP per lane. Primary antibodies: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (1 µg/ml) (Lane 2) and Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) (Cat. No. OP97) (1 µg/ml) (Lane 3). Detection: chemiluminescence.
      References
      ReferencesYang, Q., et al. 2006.Genes Dev. 20, 2820.
      Jacinto, E., et al. 2006.Cell 1, 125.
      Ali, S., et al. 2005.J. Biol. Chem. 20, 19445.
      Sarbassov, D., et al. 2005.Science 5712, 1098.
      Sarbassov, D., et al. 2004.Curr. Biol. 14, 1296.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHEK293 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunoprecipitation (10 µg/mg total protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 1-99 of human Rictor fused to GST
      ImmunogenHuman
      Clone2C8
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1122 0

      Documentation

      Anti-Rictor Mouse mAb (2C8) Certificates of Analysis

      TitleLot Number
      ST1122

      References

      Reference overview
      Yang, Q., et al. 2006.Genes Dev. 20, 2820.
      Jacinto, E., et al. 2006.Cell 1, 125.
      Ali, S., et al. 2005.J. Biol. Chem. 20, 19445.
      Sarbassov, D., et al. 2005.Science 5712, 1098.
      Sarbassov, D., et al. 2004.Curr. Biol. 14, 1296.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-September-2007 JSW
      SynonymsAnti-Rapamycin-Insensitive Companion of mTOR Mouse mAb
      ApplicationImmunoblotting (1 µg/ml)
      Immunoprecipitation (10 µg/mg total protein)
      Application Data
      Lane 1: Detection of human Rictor by immunoblotting. Sample: Whole cell lysate (40 µg) from HEK293 cells. Primary antibody: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (1 µg/ml). Detection: chemiluminescence. Lanes 2 and 3: Detection of mTOR/Rictor by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Antibody for IP: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (10 µg/mg total protein). Immunoblotting conditions: Samples: half of Rictor IP per lane. Primary antibodies: Anti-Rictor Mouse mAb (2C8) (Cat. No. ST1122) (1 µg/ml) (Lane 2) and Anti-mTOR/FRAP (Ab-2) Mouse mAb (22C2) (Cat. No. OP97) (1 µg/ml) (Lane 3). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~190 kDa Rictor protein.
      BackgroundFunctional mTOR (mammalian target of rapamycin) exists in cells in complex with other proteins, including Raptor and Rictor, which are two unrelated, cytosolic proteins that bind to and regulate mTOR. mTORC1 (mTOR complex 1) consists of mTOR, Raptor (regulatory associated protein of mTOR), and mLST8/GβL (mammalian LST8/G-protein β-subunit like protein) and functions as a nutrient/energy/redox sensor and controller of protein synthesis. Growth factor signaling, together with nutrient availability, regulates cell growth by controlling the activity of mTORC1. mTORC2 (mTOR complex 2) consists of mTOR, Rictor (rapamycin-insensitive companion of mTOR, acc # NP_689969.2), GβL, and mSIN1 (mammalian stress-activated protein kinase interacting protein 1) and functions as an important regulator of the cytoskeleton through its stimulation of F-actin stress fibers, paxillin, RhoA, Rac1, Cdc42, and PKCα. mTORC2 is also believed to regulate responses to cellular stress via the FOXO pathway. mTORC2 is also known to be the elusive PDK2, a serine/threonine kinase that phosphorylates Akt on Ser473. Biomarkers indicate that the mTOR pathway is overactive in certain cancers, suggesting that mTOR may be an attractive target for cancer therapy.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 1-99 of human Rictor fused to GST
      Clone2C8
      IsotypeIgG2a
      Specieshuman
      Positive controlHEK293 cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesYang, Q., et al. 2006.Genes Dev. 20, 2820.
      Jacinto, E., et al. 2006.Cell 1, 125.
      Ali, S., et al. 2005.J. Biol. Chem. 20, 19445.
      Sarbassov, D., et al. 2005.Science 5712, 1098.
      Sarbassov, D., et al. 2004.Curr. Biol. 14, 1296.