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MABS2288-25UL Anti-SCAP Antibody, clone 2G10

Anti-SCAP, clone 2G10, Cat. No. MABS2288, is a mouse monoclonal antibody that SREBP cleavage-activating protein (SCAP) and is tested for use in Western Blotting.

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MABS2288-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2288-25UL
      DescriptionAnti-SCAP Antibody, clone 2G10
      Alternate Names
      • Sterol regulatory element-binding protein cleavage-activating protein
      • SREBP cleavage-activating protein
      Background InformationSterol regulatory element-binding protein cleavage-activating protein (UniProt P97260; also known as SCAP, SREBP cleavage-activating protein) is encoded by the SCAP gene (Gene ID 100689048) in hamster species. SCAP is a multi-pass membrane protein with five cytoplasmic domains, eight transmembrane domains, and four lumenal domains. The C-terminal domain of SCAP mediates association with SREBPs, while transmembrane helices 2 6 comprise the sterol-sensing domain that mediates sterol-induced binding of SCAP to Insigs. SCAP is mainly localized in endoplasmic reticulum and Golgi apparatus. It requires the presence of SPRING for proper localization to endoplasmic reticulum. It mediates the activation of membrane-bound transcription factors known as sterol-regulatory element binding proteins (SREBPs) for transcribing genes encoding cholesterol biosynthetic enzymes. Low sterol concentrations are reported to trigger release of Insig and lead to a conformational change in the SSD domain (aa 284-442) of SCAP, unmasking of the ER export signal and promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi. In the Golgi, SREBPs are proteolytically processed releasing the transcription factor fragment of SREBPs from the membrane. At high sterol concentrations, formation of a ternary complex with Insig leads to masking of the ER export signal in SCAP, which promotes retention of the complex in the endoplasmic reticulum. (Ref.: Kober, DL., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(45); 28080-28091).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SCAP, clone 2G10, Cat. No. MABS2288, is a mouse monoclonal antibody that SREBP cleavage-activating protein (SCAP) and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected SCAP in Western Blotting applications (Kober D.L., et al. (2020). Proc Natl Acad Sci USA. 117(45):28080-28091).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to the first 767 amino acids of the Chinese Hamster SCAP protein combined with Ribi adjuvant.
      EpitopeN-terminal half
      Clone2G10
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2G10 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein cleavage-activating protein (SACP).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Hamster based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SCAP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~150 kDa observed; 139.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC3 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected SCAP in PC3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2288-25UL 04065267216492