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MAB131873 Anti-mCherry Antibody, clone 1C51

Anti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.

Anti-mCherry Antibody, clone 1C51 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: mCherry UniProt Number: X5DSL3
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MAB131873
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB131873
      DescriptionAnti-mCherry Antibody, clone 1C51
      Background InformationmCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. It is monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, clone1C51 is an affinity purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected mCherry in Western Blotting applications (Krajcovic, M., et. al. (2013). Mol Biol Cell. 24(23):3736-45).

      Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).

      Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).
      Biological Information
      ImmunogenHis-tagged full length recombinant mCherry.
      Clone1C51
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1C51 detects mCherry protein in multiple species.
      IsotypeIgG2aκ
      Species Reactivity
      • All
      Species Reactivity NoteAll
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • mCherry
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~32 kDa Observed; 26.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with pFin-EF1-mCherry.

      Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB131873 04054839178979

      Documentation

      Anti-mCherry Antibody, clone 1C51 Certificates of Analysis

      TitleLot Number
      Anti-mCherry, clone 1C51 3072551
      Anti-mCherry, clone 1C51 - 3195413 3195413
      Anti-mCherry, clone 1C51 - 3384834 3384834
      Anti-mCherry, clone 1C51 - 3548329 3548329
      Anti-mCherry, clone 1C51 - 3828023 3828023
      Anti-mCherry, clone 1C51 - 3905522 3905522
      Anti-mCherry, clone 1C51 - 4023638 4023638
      Anti-mCherry, clone 1C51 - 4169252 4169252
      Anti-mCherry, clone 1C51 - Q2830936A Q2830936A
      Anti-mCherry, clone 1C51 Monoclonal Antibody 3128833
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