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ABS2107-25UL Anti-phospho-CaMKII beta (Ser331)

Anti-phospho-CaMKII beta (Ser331), Cat. No. ABS2107, is a highly specific rabbit polyclonal antibody that targets Calcium/calmodulin-dependent protein kinase type II subunit beta (CaMK-II subunit beta) and has been tested for use in immunocytochemistry and Western Blotting.

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ABS2107-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2107-25UL
      DescriptionAnti-phospho-CaMKII beta (Ser331)
      Alternate Names
      • Calcium/calmodulin-dependent protein kinase type II subunit beta
      • EC: 2.7.11.17
      • CaM kinase II subunit beta
      • CaMK-II beta
      Background InformationCalcium/calmodulin-dependent protein kinase type II subunit beta (UniProt: P08413; also known as EC: 2.7.11.17, CaM kinase II subunit beta, CaMK-II subunit beta) is encoded by the Camk2b gene (Gene ID: 24245) in rat. CaM kinase II is one of the most widely studied multi-functional enzyme. It is found in high concentrations in the brain and in some regions it may constitute up to 2% of the total protein content. CaM kinase II is a complex of about 12 subunits, which exist in four differentially expressed forms (alpha, beta, gamma, and delta). CaMKII beta contains an auto-inhibitory domain (aa 283-293), a calmodulin-binding domain (aa 291-301) and a unique C-terminal association domain responsible for oligomerization. It is activated Ca2+/calmodulin and binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr-287 which turns the kinase in a constitutively active form and confers to the kinase a Ca2+-independent activity that persists even after withdrawal of calcium. Phosphorylation of Thr-287 locks the kinase into an activated state. CaM Kinase II typically recognizes the phosphorylation site sequence Arg-X-X-Ser/Thr. It prefers arginine in the P3 position, which is reported to make an electrostatic contact with Glu96 in the catalytic core.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-CaMKII beta (Ser331), Cat. No. ABS2107, is a highly specific rabbit polyclonal antibody that targets Calcium/calmodulin-dependent protein kinase type II subunit beta (CaMK-II subunit beta) and has been tested for use in immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT or mutants (Kim, K., et. al. (2015). Neuron. 87(4):813-26).

      Immunocytochemistry Analysis: A representative lot detected phospho-CaMKII beta (Ser331) in dissociated hippocampal neurons (Kim, K., et. al. (2015). Neuron. 87(4):813-26).
      Biological Information
      ImmunogenA linear peptide corresponding to 10 amino acids surrounding phopho serine 331 of rat CaM Kinase II beta.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects beta subunit of CaM Kinase II when phosphorylated on Ser331.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Camk2b
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~60 kDa observed; 60.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cells transfected with CaMKII WT or mutant S331A.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-CaMKII beta (Ser331) in HEK293T cells transfected with CaMKII WT, but not the mutant S331A.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2107-25UL 04054839505584

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