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ABS2250-25UG Anti-phospho ULK1(Ser746)

Anti-phospho ULK1(Ser746), Cat. No. ABS2250, is a rabbit polyclonal antibody that detects ULK1 phosphorylated on serine 746 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

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ABS2250-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2250-25UG
      DescriptionAnti-phospho ULK1(Ser746)
      Alternate Names
      • Serine/threonine-protein kinase ULK1
      • EC:2.7.11.1
      • Serine/threonine-protein kinase Unc51.1
      • Unc-51-like kinase 1
      Background InformationSerine/threonine-protein kinase ULK1 (UniProt: O70405; also known as EC:2.7.11.1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1) is encoded by the Ulk1 gene (Gene ID: 22241) in murine species. ULK-1 is a cytosolic serine/threonine protein kinase that is localized in cytosol and preautophagosomal structures. Under condition of starvation, it is localized to punctate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. Its protein kinase domain is localized to amino acids 16-278. It is involved in autophagy in response to starvation and acts upstream of PI3-kinase (PIK3C3) to regulate the formation of autophagophores, the precursors of autophagosomes. It acts both as a downstream effector and a negative regulator of mTORC1 via interaction with Raptor. ULK1 contains multiple phosphorylation sites, and their phosphorylation status is reported to regulate canonical autophagy. Under unstimulated conditions, it is phosphorylated at serine 637 and 757 by mTOR complex that leads to its inactivation. ULK1 is activated via phosphorylation by AMPK and also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. Despite its similarity with ULK2 in its enzymatic domain, there are major differences in their autophagy-related interactors and their post-translational and transcriptional regulators. ULK1 undergoes phosphorylation at serine 746 by receptor interacting protein kinase 3 (RIPK3) during genotoxic stress-induced alternative autophagy and this phosphorylated form is shown to localize exclusively on the Golgi and is essential for alternative autophagy, but not canonical autophagy. (Ref.: Torii, S., et al. (2020). Nat. Commun. 11: 1754).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.09% sodium azide
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho ULK1(Ser746), Cat. No. ABS2250, is a rabbit polyclonal antibody that detects ULK1 phosphorylated on serine 746 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunofluorescence
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ULK1 phosphorylated on serine 746.

      Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho ULK1(Ser746) in Immunoprecipitation applications (Satoru Torii, et al. (2020). Nat. Commun. 11(1):1754).

      Immunofluorescence Analysis: A representative lot detected phospho ULK1(Ser746) in Immunofluorescence applications (Satoru Torii, et al. (2020). Nat. Commun. 11(1):1754).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids surrounding phosphoserine 746 of mouse ULK-1.
      EpitopeC-terminal half
      Concentration0.73 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects ULK-1 phosphorylated on serine 746.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ulk1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~ 42 kDa observed; 112.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western blotting with constructs of ULK-1 phosphorylated on Serine 746.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected MBP-tagged ULK1 construct phosphorylated on serine 746, but not in non-phosphorylated construct.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2250-25UG 04065267027630