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480741 Cleaved Cdk5 Activator, p35, GST-Fusion, Bovine, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Neuronal Cdk5 Activator, p25nck5a/p35nck5a, Nck5a

480741
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, bovine cleaved Cdk5 activator (p35) fused to GST and expressed in E. coli. It is produced as a 35 kDa precursor and further cleaved into the 25 kDa form of the protein. Cleaved Cdk5 activator is an essential regulating subunit of cdk5. Aberrant Cdk5 activity has been implicated in a number of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease, where abnormal phosphorylation of tau and/or neurofilaments has been observed. One vial contains sufficient material for approximately 25 reactions.
      Catalogue Number480741
      Brand Family Calbiochem®
      SynonymsNeuronal Cdk5 Activator, p25nck5a/p35nck5a, Nck5a
      References
      ReferencesLee, K.Y., et al. 1999. Neurosci. Res. 34, 21.
      Lee, K.Y., et al. 1997. J. Biol. Chem. 272, 5622.
      Lee, K.Y., et al. 1996. J. Biol. Chem. 271, 1538.
      Tang, D., et al. 1995. J. Biol. Chem. 270, 26897.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of protein that when reconstituted with Cdk5 (Cat. No. <a href="https://./?[islink]ViewProductDetail-BySKU||SKU|EMD_BIO-219459|[/islink]">219459</a>) will transfer 1 pmol of phosphate to Histone H1 peptide (9-18) per min at 30°C, pH 7.2.
      FormLiquid
      FormulationIn 25 mM Tris-HCl, 100 µM DTT, 30% glycerol, pH 7.4.
      Applications
      Biological Information
      Specific Activity≥100,000 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      480741 0

      Documentation

      Cleaved Cdk5 Activator, p35, GST-Fusion, Bovine, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      480741

      References

      Reference overview
      Lee, K.Y., et al. 1999. Neurosci. Res. 34, 21.
      Lee, K.Y., et al. 1997. J. Biol. Chem. 272, 5622.
      Lee, K.Y., et al. 1996. J. Biol. Chem. 271, 1538.
      Tang, D., et al. 1995. J. Biol. Chem. 270, 26897.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-August-2008 RFH
      SynonymsNeuronal Cdk5 Activator, p25nck5a/p35nck5a, Nck5a
      DescriptionRecombinant, bovine cleaved Cdk5 activator (p35) fused to GST and expressed in E. coli. It is produced as a 35 kDa precursor and further cleaved into the 25 kDa form of the protein. Cleaved Cdk5 activator is an essential regulating subunit of cdk5. Aberrant Cdk5 activity has been implicated in a number of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease, where abnormal phosphorylation of tau and/or neurofilaments has been observed. One vial contains sufficient material for approximately 25 reactions.
      FormLiquid
      FormulationIn 25 mM Tris-HCl, 100 µM DTT, 30% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Specific activity≥100,000 units/mg protein
      Unit definitionOne unit is defined as the amount of protein that when reconstituted with Cdk5 (Cat. No. 219459) will transfer 1 pmol of phosphate to Histone H1 peptide (9-18) per min at 30°C, pH 7.2.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLee, K.Y., et al. 1999. Neurosci. Res. 34, 21.
      Lee, K.Y., et al. 1997. J. Biol. Chem. 272, 5622.
      Lee, K.Y., et al. 1996. J. Biol. Chem. 271, 1538.
      Tang, D., et al. 1995. J. Biol. Chem. 270, 26897.