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AP120A Goat Anti-Human IgA Antibody, IgG, and IgM, Alkaline Phosphatase

Goat anti-Human IgA Antibody, IgG & IgM, Alkaline Phosphatase detects level of Human IgA & has been published & validated for use in ELISA & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Human
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AP120A
1 mL  
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      Description
      Catalogue NumberAP120A
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Human IgA Antibody, IgG, and IgM, Alkaline Phosphatase
      Background InformationIgG is a monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

      IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.

      Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.
      References
      Product Information
      FormatAlkaline Phosphatase
      HS Code3502 90 90
      PresentationPurified goat secondary antibody IgG in lyophilized buffer containing 0.01 M Tris-HCL, 0.25 M NaCl, pH 8.0 with 15 mg/mL BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationGoat anti-Human IgA Antibody, IgG & IgM, Alkaline Phosphatase detects level of Human IgA & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern Blot:
      1:5,000-1:50,000 dilution can be used.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      HostGoat
      SpecificitySpecific for human IgG, IgM and IgA.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Enzyme-linked Immunosorbent Assay.

      ELISA:
      1:5,000-1:50,000 diltution can be used.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore lyophilized material at 2-8°C for up to 1 year after date of receipt.
      After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months.
      Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP120A 04053252473869

      Documentation

      Goat Anti-Human IgA Antibody, IgG, and IgM, Alkaline Phosphatase SDS

      Title

      Safety Data Sheet (SDS) 

      Goat Anti-Human IgA Antibody, IgG, and IgM, Alkaline Phosphatase Certificates of Analysis

      TitleLot Number
      ALKALINE PHOSPHATASE CONJUGATED AFFINITY PURIFIED SECONDARY ANTIBODY - 2165816 2165816
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 2172666 2172666
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 2228066 2228066
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 2234123 2234123
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 3287805 3287805
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 3468305 3468305
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 3492212 3492212
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 3836464 3836464
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate - 4245629 4245629
      Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate -2519259 2519259

      References

      Reference overviewPub Med ID
      Use of novel autoantibody and cancer-related protein arrays for the detection of esophageal adenocarcinoma in serum.
      Arman Kilic,Matthew J Schuchert,James D Luketich,Rodney J Landreneau,Anna E Lokshin,William L Bigbee,Talal El-Hefnawy
      The Journal of thoracic and cardiovascular surgery  136  2008

      Show Abstract       		18603076 18603076

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      Alternative Format

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies