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534331 Hypochlorous Acid Fluorescent Probe, PIS

A cell-permeable, selective & sensitive fluoro probe for HOCl. Enables HOCl detection by two-photon fluorescence microscopy in live cells & tissues.

Hypochlorous Acid Fluorescent Probe, PIS MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe

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      Overview

      Replacement Information

      Key Spec Table

      Empirical Formula
      C₁₅H₁₂N₄S
      Description
      OverviewA cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO·, ROO·, ONOO− and HO·). Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Also enables to monitor HOCl levels by two-photon image in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm) in a dose-dependent manner.
      Catalogue Number534331
      Brand Family Calbiochem®
      Synonyms1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe
      DescriptionHypochlorous Acid Fluorescent Probe, PIS
      References
      ReferencesXu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.
      Product Information
      FormBrown solid
      Hill FormulaC₁₅H₁₂N₄S
      Chemical formulaC₁₅H₁₂N₄S
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targethypochlorous acid
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      534331 0

      Documentation

      Hypochlorous Acid Fluorescent Probe, PIS SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Xu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-June-2016 JSW
      Synonyms1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe
      DescriptionA cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO•, ROO•, ONOO- and HO•. Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Dose-dependently images HOCl levels by two-photon fluorescence microscopy in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm).
      FormBrown solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₅H₁₂N₄S
      Purity≥98% by HPLC
      SolubilityDMSO (0.5 mg/ml) or DMF (0.5 mg/ml). Small yellow particles may be visible.
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesXu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.